Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Immunology > Antibody structure & function > Flashcards

Antibody structure & function Flashcards

1
Q

What can the heavy chain consist of?

A

Alpha, delta, epsilon, gamma, mu

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What can the light chain consist of?

A

Lambda, and K

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

How did they assign biological function to structure?

A

Proteolytic cleavage

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Pepsin digestion of Ig

A

F(ab’)2 and Fc fragments

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Papain digestion of Ig

A

2 x Fab and Fc

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Mercaptoethanol reduction

A

H chains and L chains

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What do the VL and VH domains contain?

A

3 hypervariable (CDR) regions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Which chain determines what kind of antibody you have?

A

Heavy chain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What defines the specificity of antibodies?

A

VH and VL regions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

How is the antigen binding site created?

A

CDRs pf H and L chains combine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Types of antigen epitopes

A

Multivalent antigen with different epitopes, multivalent antigen with repeated epitope, linear epitope or discontinuous epitope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Polyclonal

A

Antibodies secreted by different B cell lineages e.g. serum antibodies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

How are monoclonal antibodies derived?

A

From a single plasma cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What can monoclonal antibodies be used for?

A

Treating disease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What are the 4 types of therapeutic monoclonal antibody?

A

Mouse, chimeric, humanised, human

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What are the IgG subclasses?

A

IgG1, IgG2, IgG3, IgG4

17
Q

How do the IgG subclasses differ?

A

Arrangement of disulphide bonds linking the heavy chains

18
Q

Which IgG subclass is most susceptible to proteolytic cleavage but has greater capacity to bind C1q and activate complement

A

IgG3

19
Q

What antibodies are good at activating and fixing complement

A

IgM and IgG3

20
Q

What is the first complement

A

C1q (consists of 18 peptide chains)

21
Q

What antibodies are bad at activating and fixing complement?

A

IgD, IgG4, IgE

22
Q

What does IgM contain that is required for polymerisation?

A

J chain

23
Q

How many antigen binding sites does IgM contain?

A

10

24
Q

What is IgA important for?

A

External secretions (mucosal surfaces e.g. gut, lungs)

25
Q

What is the structure of IgA?

A

Dimeric with J chain and secretory component (composed with Ig-like domain) to link 2 chains together

26
Q

What is the secretory component needed for?

A

Stability

27
Q

What is required for Ig transport across epithelia?

A

Poly-Ig receptor

28
Q

What is the major antibody class synthesised at mucosal sites

A

IgA

29
Q

Where is IgE found

A

Bound to receptors on mast cells

30
Q

What is a mast cell?

A

Cell filled with basophil granules (found in connective tissue) - releases histamine and other substances during inflammatory and allergic reactions.

31
Q

Why is IgE important in allergic reactions?

A

Cross-linking by antigen (allergen) causes release of allergic mediators

32
Q

What is an allergen?

A

Antigen that stimulates IgE

33
Q

What do Fc receptors do?

A

Allow cells to be activated by antibody bound to antigens/ pathogens

34
Q

What is the high affinity receptor for IgG1 and IgG3

A

Fc(gamma)RI - consisys of alpha and gamma chain

35
Q

What part of the antibody does Fc(gamma)RI bind?

A

The lower hinge and constant heavy2 of IgG3

36
Q

What is the role of Fc receptors on phagocytes?

A

Trigger the uptake and destruction of antibody-coated pathogens

37
Q

How can you distinguish an inhibitory Fc receptor?

A

ITIM domain on cytoplasmic side

38
Q

What does the B cell receptor consist of?

A

Membrane bound Ig with Igalpha and Igbeta chains

39
Q

After how many days is there a primary antibody response?

A

14

Immunology (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions