Antibody Qs 8-14

Question 1

Antibody affinity for antigen depends on:

Answer 1

The complementary shape and charge of each antibody V region for its antigen epitope.

Question 2

Avidity:

Answer 2

Refers to the strength of iteractions between a multivalent antibody and a bultivalent antigen.

Question 3

A colleague sends you an antibody to polio virus capsid protein. You preform equilibrium dialysis on the antibody to measure its affinity. Plotting r/c versus r gives you a curved line with K=2.5 X 10ˆ8 L/mole and an r intercept of 4. From these results, you conclude that the antibody is probably:

Answer 3

IgA anti-polio virus.

Question 4

Allotypic determinants are:

Answer 4

Amino acid differences encoded by different alleles for the same H or L chain locus.

Question 5

Which of the following is NOT a characteristic of IgG?

Answer 5

It is the largest of all Igs.

Question 6

What are the characteristics for IgG?

Answer 6

• It contains 2g and 2L chains
• It crosses placenta
• It is the predominant immunoglobulin in blood
• Lymph and peritoneal fluid
• Its L chain are either k or l

Question 7

Human serum IgA is isolated and injected into a rabbit. The rabbit anti-IgA antibodies will react against all of the following EXCEPT human:

Answer 7

Secretory component.