Antibody 11-20 Flashcards
Allotypic determinants are:
Amino acid differences encoded by different alleles for the same H or L chain locus.
Which of the following is NOT a characteristic of IgG?
A. it contains 2 g and 2 L chains.
B. it crosses the placenta.
C. it is the predominant immunoglobulin in blood, lymph, and peritoneal fluid.
D. it is the largest of all the Igs.
E. its L vhains are either k or l.
D. it is the largest of all the Igs.
Human serum IgA is isolated and injected into a rabbit. The rabbit anti-IgA antibodies will react against all of the following EXCEPT human:
A. a chain.
B. IgG.
C. k chain.
D. l chain
E. secretory component.
E. secretory component.
You have purified some Fab from an IgG myeloma protein. Under appropriate conditions, you could use this Fab to generate antibodies to:
The idiotype of this myeloma.
The Ig isotype which would be most important for neutralizing polio virus before it could infect intestinal cells would be:
Secretory IgA.
which of the following changes to a serum IgM antibody molecule would definitely DECREASE its avidity?
A. increase noncovalent antigen-antibody interactions in the CDR.
B. Remove the secretory component.
C. Replace the Fc portion of the mu chains with the Fc portion of alpha chains.
D. Replace V_H and V_L framework regions with those from a different antibody.
E. Use limited enzyme digestion to make Fab fragments.
E. Use limited enzyme digestion to make Fab fragments.
IgA can be secreted from the body because it:
Binds poly-Ig receptor on mucosal epithelial cells.
The ability to make antibody with the same antigen specificity but different Fc regions:
Increases the effector functions of Ig molecules.
Allergy symptoms are produced when antigen binds to IgE on FcR on:
Mast cells.
One amino acid difference in the Fc region of different human g chains is the epitope recognized by anti-
allotype.
