Antibody 1-10

Question 1

An antibody Fab contains:

Answer 1

A. complementary determining regions
B. H and L chain variable regions.
C. one antigen binding region.
D. one H-L interchain disulfide bond.

E. all of the above ( in MCQs this is the correct one)

Question 2

Myeloma proteins are:

Answer 2

Homogeneous antibody molecules secreted by plasma cell tumors.

Question 3

The regions of the antibody molecule which contribute MOSTto the affinity of the antibody for antigen are the:

Answer 3

CDR (complementarity-determining region)

Question 4

Antibody Fc fragments contain:

Answer 4

Complement-binding sites.

Question 5

The immunoglobulin isotype is determined by the:

Answer 5

H chain constant region.

Question 6

Which statement about antigen epitopes is FALSE?

A. an epitope may be shared by two different antigens.
B. a protein molecule usually contains multiple epitopes.
C. B cells bind only processed antigen epitopes.
D. epitopes may be linear (composed of sequential amino acids) or assembled by protein folding from amino acids far apart in the protein primary amino acid sequence.
E. some epitopes are more immunogenic than others.

Answer 6

C. B cells bind only processed antigen epitopes.

Question 7

An example of an antigen epitope from an infectious organism would be:

Answer 7

A peptide on the surface of a virus capsid protein

Question 8

Antibody affinity for antigen depends on:

Answer 8

The complementary shape and charge of each antibody V region for its antigen epitope.

Question 9

Avidity:

Answer 9

Refers to the strenght of interactions between a multivalent antibody and a multivalent antigen.

Question 10

A colleague sends you an antibody to polio virus capsid protein. You perform equilibrium dialysis on the antibody to measure its affinity. Plotting r/c versus r gives you a curved line with K=2.5 X 10^8 L/mole and an r intercept of 4. From these results, you conclude that the antibody is probably:

Answer 10

IgA anti-polio virus.