Antibodies As Therapeutic Entities

Question 1

How do you get polyclonal antibodies?

Answer 1

Often immunise donor animal with some form of adjuvant, do this three times and try to get the maximum response. Take a small blood sample, then do affinity purification with antibodies that stick to your antigen

Question 2

What is omab?

Answer 2

Mouse

Question 3

What is zmab?

Answer 3

Chimeric

Question 4

What is zumab or xizumab?

Answer 4

Human antibody

Question 5

What is umab?

Answer 5

Fully human

Question 6

With ximab, what sections can be switched?

Answer 6

Fab sections, do fab swaps for light and heavy chain

Question 7

What do you swap for zumabs?

Answer 7

CDRs, have transferred in an entire variable domain

Question 8

What is good about transgenic mice?

Answer 8

They have a human immune system so they can make human antibodies

Question 9

How did rituximab work?

Answer 9

Was a depletion antibody - anti cd20 that depleted B cells
Would kill off stuff to make B cells numbers in peoples blood drop, was good for cancer, now used in rheumatoid arthritis

Question 10

What is good about bispecifics?

Answer 10

One arm has a different specificity to another, can use this as attaching two things to one another