Antibodies

Question 1

Other term for antibodies

Answer 1

Immunoglobulins

Question 2

End stage reaction of Humoral Immune Response

Answer 2

Antibody production

Question 3

Type of immune response wherein antibody production occurs

Answer 3

Humoral Immune Response

Question 4

Same with antibody classes; Lies on the heavy chain structure of the antibody

Answer 4

Isotype

Question 5

Represents the genetically determined differences in the antibodies between individuals specifically in the amino acid sequence in the constant region

Answer 5

Allotype

Question 6

Pertains to the variable region of the antibody

Answer 6

Idiotype

Question 7

Recognizes different specific epitope present

Answer 7

Idiotype

Question 8

Heavy Chain’s site of synthesis

Answer 8

Chromosome 14

Question 9

Kappa Light Chain’s site of synthesis

Answer 9

Chromosome 2

Question 10

Lambda Light Chain’s site of synthesis

Answer 10

Chromosome 22

Question 11

Found between CH1
and CH2 – flexible region of the
antibody so that it can squeeze through the tissues

Answer 11

Hinge Region

Question 12

A glycoprotein that is responsible for linking or bridging monomers together; Found in antibodies consist of 2 or more monomers

Answer 12

J Chain (Joining chain)

Question 13

Two terminal regions of Immunoglobulins

Answer 13

1. Carboxyterminal
2. Aminoterminal

Question 14

This bond holds light and heavy chains together

Answer 14

Covalent disulfide bonds

Question 15

This links heavy chains together

Answer 15

Disulfide linkages

Question 16

Four polypeptide chains of antibody

Answer 16

1. 2 identical light chains
2. 2 identical heavy chains

Question 17

This terminal region dictates the allotype

Answer 17

Carboxyterminal

Question 18

This is where the crystallizable fragment (Fc) is located (constant region)

Answer 18

Carboxyterminal

Question 19

This terminal region determines the idiotype; variable region is located

Answer 19

Aminoterminal

Question 20

This specifically binds to the epitope of the antigen

Answer 20

Hypervariable portion of the variable region

Question 21

Varying antibody specificity

Answer 21

Aminoterminal

Question 22

Treatment with PAPAIN will produce which fragments?

Answer 22

1 Fc fragment
2 Fab fragment

Question 23

Fc fragment when treated with papain are involved in the following Ig biologic function:

Answer 23

▪ Involve in Complement Fixation
▪ Placental transfer of antibodies
▪ Binding site for cells

Question 24

What happens to 2 Fab fragments when antibody is treated with Papain?

Answer 24

Fab fragments cannot agglutinate/precipitate but still able of antigen binding.

Question 25

Treatment with Pepsin will produce which fragments?

Answer 25

2 Fab fragments

Question 26

What happens to Fc fragments when antibody is treated with Pepsin?

Answer 26

The Fc fragment is digested

Question 27

True or False: In pepsin treatment of antibody, The two Fab fragments has two antigen combining sites (Bivalent), therefore, it is capable of antigen binding, precipitation and agglutination.

Answer 27

True

Question 28

How many domains of immunoglobulin are there?

Answer 28

Five domains

Question 29

What are the domains of immunoglobulins

Answer 29

1. Variable region 2. CH2 3. CH3 4. CL 5. Hinge Region

Question 30

In domains of immunoglobulin, which domain is Responsible for the specificity of the antibody?

Answer 30

Variable region

Question 31

In domains of immunoglobulin, which domain binds with complement?

Answer 31

Constant Heavy Chain 2 (CH2)

Question 32

What antibodies are involved in the activation of Classical Pathway?

Answer 32

IgG and IgM

Question 33

In domains of immunoglobulin, which domain responsible for Cytotropic reaction?

Answer 33

Constant Heavy Chain 3 (CH3)

Question 34

This domain connects with receptors on cell membrane

Answer 34

Constant Heavy Chain 3 (CH3)

Question 35

This domain is either Kappa or Lambda

Answer 35

Constant Light Chain

Question 36

Percentage of Kappa Antibody Light chain

Answer 36

65%

Question 37

Percentage of Lambda Antibody Light chain

Answer 37

35%

Question 38

This domain is found between the CH1 and CH2 type of antibody

Answer 38

Hinge Region

Question 39

Which complement protein does CH2 bind?

Answer 39

C1 specifically C1q

Question 40

The domain where Fab portion is located – the binding site of the epitope of antigens

Answer 40

Variable region

Question 41

Why is flexibility important for Antibody?

Answer 41

It enables them to squeeze through or penetrate to the membrane

Question 42

Which amino acid is attributed to the flexibility of the antibody?

Answer 42

Proline