Anatomy

Question 1

Why are cells in histology and pathology fixed?

Answer 1

Prevents degradation of the cells

Helps maintain the architectural organisation of cells and tissues

Question 2

Haematoxylin

Answer 2

N- Purple

Question 3

Eosin

Answer 3

N, C, CT ALL PINK

Question 4

H&E

Answer 4

N- Purple
C- Pink
CT- Pink

Question 5

Haematoxylin and van Gieson

Answer 5

Grey/Blue- N
Green/Yellow- C
Collagen (red)- CT

Question 6

Masson’s trichrome

Answer 6

N- Purple
C- Brick red
CT- Green/Blue

Question 7

Elastic van Gieson

Answer 7

N- Grey/Blue
C- Green/Yellow
CT- Collagen (red)
Elastic fibres- black

Question 8

Bright field or light microscope

Answer 8

Used to produce most of the photomicrographs in the histology course

Question 9

Flouresence microscope

Answer 9

Used to analyse specimens containing fluorescent molecules under UV light

Question 10

Confocal scanning microscope

Answer 10

Laser, single area of specimen at a time. Computer image processing optically slices the image to 1mm sections (3D image)

Question 11

SEM and TEM

Answer 11

Beam of electrons, cathode electrons attached to an anode image viewed on a phosphor screen

Light microscope magnifying glass x 2000
TEM (Trans- all the way through)
Electrons- absorbed/transmitted through tissue
SEM Electrons reflected