Analytical Methods Flashcards
Lipid workup begins with what?
Measurement of Total Serum Cholesterol
What are we looking for in Cholesterol Measurement?
Absorbance (~500nm)
Linear Assays (600-700mg/dL)
Color Development
Bilirubin, Ascorbic Acid, and Hemoglobin are responsible for what?
Interference in Cholesterol Measurement
What is important about the Cholesteryl Ester Hydrolase Method?
Most common
Cholesteryl Esterase
Cholesterol Oxidase
Horseradish Peroxidase used to detect color change
Which Measurement is fairly specific and does not detect Glucose or Phospholipids?
Triglyceride Measurement
What occurs first in both methods of measurement?
Enzymes cleaving Fatty Acids from Glycerol
What is important in Triglyceride Measurement?
Bacterial Lipase
Glycerokinase
Glycerophosphate Oxidase
Horseradish Peroxidase to detect color change
Why do we no longer use HDL-C Measurement?
Interference from elevate Triglyceride Levels
What is the method we use now and why is it important?
Homogenous Assays
Automation
Uses Polymers, Detergents, and Enzymes
Highly precise and reasonably accurate
What is the Friedewald Calculation?
LDL-C = [TOT CHOL] - [HDL-C] - Trigly/5
When should the Friedewald Equation not be used?
Tryglercide concentration above 400
Significant number of Chylomicrons
Patient has Dysbetalipoproteinemia
What is the Gold Standard of measurement for LDL-C?
Beta Quantification
Ultracentrifuge
Not widely used
