ANALYSIS AND SEPARATION Flashcards
is the study of the separation, identification, and
quantification of the chemical components of
natural and artificial materials.
analytical chemistry
is the simplest physical property we can exploit in a separation.
size
In a _______ we separate a particulate interferent from soluble
analytes using a filter with a pore size that will retain the
interferent.
filtration
The solution that passes through the filter is called
the
filtrate
material retained by the filter is
retentate
three types of membrane filters
centrifugal filter
membrane syringe filter
disposable filter
is another example of a separation technique in which size is used
to separate the analyte and the interferent
dialysis
is made using cellulose and fashioned into tubing, bags, or
cassettes.
dialysis membrane
is widely used in the
analysis of polymers, and in biochemistry, where it is used for the
separation of proteins.
size-exclusion chromatography
If the analyte and the interferent have different masses or
densities, then a separation using _______ may be
possible.
centrifugation
One widely used technique for preventing an
interference is to bind the interferent in a strong,
soluble complex that prevents it from interfering in the
analyte’s determination.
Masking
When the analyte and the interferent are miscible liquids, separation
by _______ is possible if their boiling points are significantly different.
distillation
It involves the immediate change of liquid state to vapours state
and condensing of vapours by cooling. This method is only
applicable for liquids having a minimum boiling point of difference
of 25 degrees Celsius
simple distillation
The liquids whose boiling point difference is more than 25 degree
Celsius or the liquids which have similar boiling points performs this
process. I
fractional distillation
It is a process involving thermally responsive compounds
(compounds that are sensitive to heat). It requires low
heating at temperatures because the temperature of the
steam can be controlled as compared to surface
temperature. E
steam distillation