Anaerobes Flashcards
What should always accompany anaerobe cultures
aerobe cultures/ specials
Bacteroides fragilis: GS, colony morphology
GNR
grey, glistening
Clostridium perfringens: GS, hemolysis
GPR, boxy, boxcar, double zone of hemolysis
Prevotella: morphology, UV
black colonies
red under UV
Fusobacterium: GS, UV
GNR, pointy
green/ chartreuse under UV
What other organism can also be chartreuse under UV
C. diff
What two organisms are aerotolerant
Propionobacterium/ cuty
actinomyces
What organisms should you workup on anaerobic bench
Bacteriodes fragilis, Clostridium, Prevotella, Fusobacterium, actinomyces, propionobacterium/ cuty
If there are 3 or more anaerobes on the same specimen, what should you pull out to fully ID
B. fragilis
Clostridium
What are the anaerobic conditions
85% N2
10% CO2
5% H2
Actinomyces colony morphology
molar tooth, sulfur granules
If an organism is R to Colistin and S to Vanc it is likely
GP
If an organism is S to Colistin and R to Vanc it is likely
GN
What is an exception for an organism that is R to vanc
lactobacillus- GP but R to vanc
What organism is S to SPS
Peptococcan
What is the best source of specimen for anaerobic culture
deep tissue or aspirate
What specimen should be rejected in anaerobes
skin swab
What are the agars used in anaerobes
CDCANA
CNA
BBE- B. fragilis
LKV- Prevotella, B. fragilis
What extra media do joints get and why
CMG- grows propionobacterium after 2 weeks
Propionobacterium: biochemicals, O2 requirements, growth length
Catalase and indole +, aerotolerant, 2 weeks to grow
What 2 organisms are strict anaerobes
Clostridium and fusobacterium
B. urealyticum morphology
pits the agar
Breadcrumb or ground glass
Fusobacterium
Black pigmented colonies
Prevotella
