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Micro Lab > Acid Fast Bacteria > Flashcards

Acid Fast Bacteria Flashcards

1
Q

What do AFB have in their cell walls?

A

extra lipids that are R to dyes

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2
Q

What is in LJ agar, what does it grow

A

eggs, potato, malachite green, Mycobacterium

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3
Q

What is in Middlebrook 7H10, what does it grow

A

malachite green, albumin, suppresses non mycobacterium

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4
Q

What is in Middlebrook 7H11, what does it grow

A

caesin hydrosylate
grows M. tuberculosis

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5
Q

What does malachite green do

A

suppresses GPOs

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6
Q

What does albumin do in AFB agars

A

suppresses non mycobacterium

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7
Q

NAOH

A

decontaminant
kills normal flora that might be present

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8
Q

NALC

A

produces mucolytic agent to break up mucus
N-acetyl-L-cysteine
digestant

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9
Q

NALC and NaOH are used for

A

sterilizing AFB specimen to help grow AFB

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10
Q

What does the Auramine rhodamine stain look like

A

yellow organism
black background

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11
Q

What does the Kinyoun stain look like

A

pink organism
blue background

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12
Q

What mycobacterium is the most sever

A

M. tuberculosis

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13
Q

What is M. marinum associated with

A

water, skin, fish

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14
Q

What is M. xenopi associated with

A

hot water systems

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15
Q

Photochromogens

A

produce cartoene pigment after exposure to light
M, simae
M. kansaii
M. marinum

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16
Q

Scotochromogens

A

Produce pigment in light or without light
M. scrofulaceum

17
Q

Non photochromogens

A

do not produce pigment in light
M. tuberculosis
M. bovis
M. avium

18
Q

What does M. haemophilum need to grow

A

Choc, soft tissue, RT

19
Q

M. tuberculosis: GS, biochemicals, lab test, pop

A

Acid fast slender, curved, cording
niacin +
nitrate +
PPD skin test
Malnutrition, alcoholism, homelessness, incarceration, immunosuppression, AIDs

20
Q

What organisms are in the mycobacterium avium complex

A

M. tuberculosis
M. bovis
M. africanum
M. canetti
M. microti

Micro Lab (5 decks)

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