Anabolic Metabolism: Nuclei Acids and DNA

Question 1

H-bonding in DNA + DNA binding proteins

Answer 1

Thymine-Adenine (2 H-bonds) (TA-TOO)
C-G (3 h bonds)
Histones are positively charged - associate with Negative DNA

Histones can be acetylated to make less positive - looser association with DNA

Question 2

DNA vs RNA

Answer 2

DNA has deoxy ribose (missing -OH on C2)

Question 3

Epigenetic Changes

Answer 3

Histone Acetylation - removes + charge from NH4 group to give more access to transcription

DNA methylation - turns gene on or off

Question 4

Deamination and Tautamerization

Answer 4

Deamination - point mutation that may occur which is not corrected before replication

causes: C-U, G-Xanthine, A-Hypoxanthine, T-doesnt deaminate

Tautomerization - causes bases to mismatch

A-T -> A-C