amoebas Flashcards
The most important feature that separates amebas from the other groups of unicellular Protozoa is
the means by which they move.
Amoebas are equipped with the ability to extend their cytoplasm in the form
of pseudopods (false feet), which allows them move within their environment.
Trophozoites
the form that feeds, multiplies, and possesses pseudopods
Cysts
the nonfeeding stage characterized by a thick protective cell wall designed to protect the parasite from the harsh outside environment
The morphologic conversion from the cyst form into the trophozoite form, occurs in the ileocecal area of the intestine.
Excystation
The conversion of trophozoite to cysts.
Encystation
often show motility of the amebic trophozoites.
Saline wet preparations
The internal cytoplasmic, as well as the nuclear structures, may be more readily seen with the use of
iodine wet preparations
In the United States, amebiasis is often found in
immigrants from and people who have traveled to underdeveloped countries.
The trophozoite exhibits rapid, unidirectional, progressive movement.
Entamoeba histolytica troph
The nucleus contains a small central mass of chromatin known as a karyosome (karyosomal chromatin).
Entamoeba histolytica troph
The karyosome is surrounded by peripheral chromatin.
Entamoeba histolytica troph
Entamoeba histolytica troph no. of nuclei
1
Entamoeba histolytica trophcytoplasmic inclusion
ingested rbc
Entamoeba histolytica troph cytoplasm
finely granular
Entamoeba histolytica cyst shape
spherical to round
Entamoeba histolytica cyst no. of nuclei
1-4
Entamoeba histolytica cyst cytoplasmic inclusion
- chromatoid bars, rounded ends in young cyst
- diffused glycogen mass
Young cysts characteristically contain unorganized chromatin material that transforms into squared or round ended structures call chromatoid bars, defined as structures that contain condensed RNA material.
Entamoeba histolytica
RBCs, bacteria, yeast,
and other debris are
not found in the cyst
stage.
Entamoeba histolytica
Entamoeba histolytica lab diagnosis
- A special medium known at TYI-S- 33 supports E. histolytica in culture.
- Methods currently available include antigen tests, enzyme- linked immunosorbent assay (ELISA), indirect hemagglutination (IHA), gel diffusion precipitin (GDP), and indirect immunofluorescence (IIF).
AT ELISA IHA GDP IIF