Amoeba Flashcards
What is the outer membrane of amoeba called
Periplast
The outer region of the cytoplasm in amoeba
Ectoplasm
The inner region in the cytoplasm of amoeba
Endoplasm
Unicellular eukaryotes with membrane bound organelles
Amoeba
Responsible for the osmoregulation of amoeba
Contractile vacuole
An amoeba can have how many nucleus
2 or more
What are the food sources of amoeba
Bacteria and protists
What is the nutrition of amoeba called
Holozoic
Process where the entire organism engulfs the food it plans on eating up
Phagocytosis
All amoeba are commensals except for
Entamoeba histolytica
All amoeba are found in the colon except
Entamoeba gingivalis
Small karyosome at or near the center
Entamoeba
Large, irregular karyosome attached to nuclear membrane it has nor peripheral chromatin and it forms cysts
Endolimax
Large karyosome surrounded by achromatic granules it has no peripheral chromatin
Iodamoeba
Generally binucleate; central particulate karyosome
Dientamoeba
What are the 3 genera of amoeba that inhibit the intestinal tract
Entamoeba, Endolimax and Iodamoeba
The only amebic specie that is capable of invading tissues and causing disease
Entamoeba histolytica
Most invasive and pathogenic kind of amoeba
Entamoeba histolytica
The most common method for diagnosing E. histolytica
Microscopic identification of cysts and trophozoite
Shows chromatid body with a bluntly rounded end
Entamoeba histolytica
How many nuclei does E. histolytica has
4
Pseudopods in E. histolytica is
Finger like
Pseudopods in Entamoeba coli is
Blunt or rounded
What is the motility of Entamoeba histolytica
Progressive and directional
What is the motility of Entamoeba coli
Sluggish
Where is the karyosome of Entamoeba histolytica located
Center
Location of Entamoeba coli karyosome
Eccentric
MOT of Entamoeba histolytica
Fecal-oral transmission
Life cycle of Entamoeba histolytica consists of 2 stages
Invasive trophozoite and infective cyst
Have the ability to colonize or invade the large bowel
Trophozoite
E. histolytica multiplies by
Binary fission
Can survive in a moist environment for weeks
E. histolytica cyst
Acute disease characterized by bloody diarrhea with abdominal cramping
Amebic dysentery
Infection of the intestines which causes diarrhea containing blood or mucus
Dysentery
Hexagonal structures formed formed from the breakdown of eosinophils and maybe seen in the stools
Chargot-Leyden crystals
Mimic ulcerative colitis
Amebic colitis
Develops a small, pinpoint mucosal ulcerations and expand within the submucosa to form flask-shaped ulcers
Amebic colitis
Most common form of extra intestinal amebiasis
Amebic liver abcess
Amount of stool specimens collected for the diagnosis of E. histolytica
3 stool specimens
Fresh stool should be examined within how many minutes
30 mins
Observe trophozoite motility
DFS with saline
Differentiates Entamoeba from WBC
Saline and Methylene blue
Nucleus and karyosome can be observed by
Iodine and saline
Drug of choice for Entamoeba histolytica
Metronidazole
It has a spherical nucleus with distinct nuclear membrane
Entamoeba
Has a vesicular nucleus with large and irregular shaped karyosome
Endolimax
Large chromatin rich karyosome surrounded by a layer of achromatic globules
Iodameoba
Morphologically similar to Entamoeba histolytica but differ in DNA and RNA
Entamoeba dispar
They are first detected in sewage
Entamoeba moshkovskii
Physiologically unique-osmotolerant and able to survive at 0-41 degrees celsius
Entamoeba moshovskii
Has been called small race Entamoeba histolytica
Entamoeba hartmanni
Cosmopolitan in distribution and is more common than other human amoeba
Entamoeba coli
A parasite found in the intestines of pigs and monkeys
Entamoeba polecki
It can be found in the oral cavity
Entamoeba gingivalis
Trophozoite measurement of E. gingivalis
10-20 micrometer
It moves quickly because it has numerous pseudopods
Entamoeba gingivalis
Amoeba that has no cyst stage
Entamoeba gingivalis
The smallest amoeba to infect humans
Endolimax nana
How many nuclei does Endolimax nana have
4
Cyst is seen as cross eyed
Endolimax nana
What is found in formed stools
Cysts
What is found in liquid or semi formed stools
Trophozoite
It is added to enhance nuclear detail and avoid confusion of amoeba with other blood cellular elements
Methylene blue
Trophozoites are best demonstrated by using what technique
Direct fecal smear
Usually found in contact lenses solution
Acanthamoeba keratitis
An aquatic organism that is found in myriad of natural and artificial environments
Acanthamoeba species
Thorn-like appendages and a highly resilient cyst stage
Acanthamoeba species
Opportunistic ocular surface pathogens causing keratitis
Acanthamoeba keratitis
Major causative agent for Primary Amebic Meningoenciphilitis
Naegleria species
Most commonly acquired by diving or swimming during hot weather
Naegleria species
Thermophilic organism that throve best in hot springs
Naegleria species
The only species of Naegleria to infect people
Naegleria fowleri
Usually found in CSF and tissue
Naegleria fowleri trophozoites
Found in CSF
flagellated forms of Naegleria fowleri
Able to survive in elevated temperatures and reproduces rapidly in temperatures above 30 celsius
Naegleria fowleri