AMOEBA Flashcards
MOST INVASIVE AND ONLY THAT CAUSED COLITIS AND LIVER ABSCESS
ENTAMOEBA HISTOLYTICA
IS STAGE OF E. HISTOLYTICA
CYST
DEFINITIVE HOST OF E. HISTOLYTICA
HUMANS *NO ANIMAL RESERVOIR
STAGE IN E. HISTOLYTICA THAT CAUSES AMOEBIASIS
TROPHOZOITE
VIRULENCE FACTORS CAUSED BY E. HISTOLYTICA
ADHERENCE
INVASION
ENDOTOXINS
2 TYPES OF AMOEBIASIS FOR SYMPTOMATIC PX.
ACUTE DYSENTERIC & CHRONIC NON-DYSENTERIC
EXTRA-INTESTINAL AMOEBIASIS
AMOEBIC LIVER ABSCESS (ALA)
DIAGNOSIS OF ALA
ANCHOVY PASTE– ASPIRATION OF NON-ODOROUSM REDDISH BROWN
IN DX., TROPHOZOITE & CYSTS CAN BE SEEN IN WHAT?
TROPHOZOITE- LOOSE STOOL
CYST- SOLID STOOL
TREATMENT FOR INVASIVE FORM OF AMOEBIASIS
METRONIDAZOLE
TREATMENT FOR LUMINAL FORMS OF E. HISTOLYITICA
IODOQUINOFONUM, PAROMOMYCIN, DILOXANIDE
TX FOR EXTRAINTESTINAL AMOEBIASIS
METRONIDAZOLE + LUMINAL FORM DRUGS
COMMENSAL AMOEBAS (NON-INVASIVE)
E. DISPAR E. MOSHKOVSKII E. HARTMANI E. COLI E. POLECKI E. CHATTONI E. GINGIVALIS ENDOLIMAX NANA IODAMOEBA BUTSCHII
COMMENSAL AMOEBA THAT DOES’NT HAVE CYSTIC STAGE
E. GINGIVALIS
AMOEBA THAT IS TRANSMITTED THRU KISSING AND IS FOUND IN MOUTH
E. GINGIVALIS
MORPHOLOGICALLY SIMILAR TO E. HISTOLYTICA BUT HAVE DIFFERENT DNA & rRNA
E. DISPAR
PHYSIOLOGIC DISTINGUISHING CHARACTERISTIC OF E. MOSHKOVSKII
(ISOLATE) OSMOTOLERANT (CAN GROW AT ROOM TEMP, SURVIVE AT 0-40ºC)
DETECTED IN SEWAGES AND INDISTINGUISHABLE FR. E. DISPAR & E. HISTOLYTICA MORPHOLOGICALLY
E. MOSHKOVSKII
CHARACTERISTIC OF E. HARTMANI
DOES NOT INGEST RBC
MORPHOLOGY OF E. HARTMANI
SIMILAR TO E. HISTOLYICA BUT SMALLER
CYST: QUADRINUCLEATED; ROD-SHAPED
MORPHOLOGY OF E. COLI
LARGER TROPHOZOITE
CYST: DIFFERENTIATED FR. E. HISTOLYTICA
MORE GRANULAR CYTOPLASM
MORE NUCLEI (8)
SPHINCTER LIKE CHROMATOIDAL BODIES
IT IS THE MORE COMMON AMOEBA
E. COLI
FOUND IN INTESTINE OF PIGS & MONKEYS
E. POLECKI
E. CHATTONI IS FOUND IN WHERE
MONKEYS & APES
IT HAS THE SAME FREQUENCY AS TO E. COLI
ENDOLIMAX NANA
MOTILITY OF E. POLECKI & E. CHATTONI
SLUGGISH MOTILITY
COMMENSAL AMOEBA THAT IS UNINUCLEATED WITH LARGE GLYCOGEN BODY THAT STAINS DARK BROWN WITH IODINE
IODAMOEBA BUSHKII
CAUSATIVE AGENT OF ACANTHOEMABA KERATITIS & GRANULOMATOUS AMEBIC ENCEPHALITIS
ACANTHAMOEBA SPP.
CA OF PAM (PRIMARY AMOEBIC MENINGOENCEPHALITIS)
NAEGLERIA FOWLERI
INFECTIVE STAGE OF ACANTHAMOEBA SPP.
TROPHOZOITE
STAGES OF ACANTHAMOEBA
CYST & TROPHOZOITES
* NO FLAGELLATED STAGE
ACANTHAMOEBA HABITAT
CAN SURVIVE IN CONTACT LENSES CLEANING SOLUTIONS
HOW DO ACANTHAMOEBA REPRODUCE
BINARY FISSION
HOW DO THE TROPHOZOITE STAGE OF ACANTHAMOEBA REPLICATE
BY MITOSIS (NUCLEAR MEMBRANE DOES NOT REMAIN INTACT)
MORPHOLOGY OF TROPHOZOITE OF ACANTHOMEBA
ACTIVE W/ PROMINENT THORN-LIKE APPENDAGES – “ACANTHOPODIA”
WHERE DOES THE TROPHOZOITE STAGE OF ACANTHAMOBA FEEDS
FEEDS ON GRAM-NEGATIVE BACTERIA, BLUE-GREEN ALGAE YEASTS
MOST AFFECTED AREAS OF THE BRAIN IN GAE
POSTERIOR FOSSA STRUCTURES
THALAMUS
BRAINSTEM
MAJOR DIFFERENTIAL TO BE RULED OUT IN DX AK
FUNGAL & HEPATIC KERATITIS
DX. OF AK
EPITHELIAL BIPSY WITH CORNEAL SCRAPING
DX OF GAE
USUALLY POSTMORTEM
SPECIES TO HAVE CAUSED AK
A. CASTELLANI A. CULBERTSONI A. HUTCHETTI A. POLYPHAGA A. RHYSOIDES
ONLY CURATIVE TX FOR AK & GAE
SURGICAL EXCISION OF INFECTED CORNEA W/ SUBSEQUENT TX.
WHAT SHOULD BE AVOIDED IN TREATING AK & GAE
TOPICAL CORTICOSTEROIDS
PROCEDURE OF CHOICE IN AK & GAE
DEEP LAMELLAR KERATECTOMY
2 FORMS OF TROPHOZOITE OF NAEGLERIA FOWLERI
AMOEBOID
AMOEBOFLAGELLATE
3 STAGES OF NAEGLERIA SPP.
CYST
TROPHOZOITE
FLAGELLATED
ROUTE OF ENTRY OF N. FOWLERI
THRU OLFACTORY BULB
ONLY NAEGLERIA SPECIES TO HAVE CAUSED DISEASE IN HUMANS
NAEGLERIA FOWLERI
WHERE DO NAEGLERIA THRIVE BEST
THERMOPHILIC– HOT SPRINGS & WARM AQUATIC AREAS
SYMPTOMS OF PAM ARE INDISTINGUISHABLE FROM
BACTERIAL MENINGITIS
TX OF PAM
AMPHOTERICIN B + CLOTRIMAZOLE
PROPHYLAXIS OF N. FOWLERI
EASILY KILLED BY CHLORINATION AT 1 PPM OR HIGHER
