ammonia Flashcards
1
Q
- Formed through the deamination of amino acids during protein metabolism.
- Removed from the circulation and converted to urea in the liver
A
ammonia
2
Q
ammonia method
NH3 + bromophenol = blue color
A
spectrophotometric
3
Q
(?) is toxic; however ammonia is present in the plasma in low concentrations
A
free ammonia
4
Q
ammonia method
- Enzymatic reaction of NH, 2-oxoglutarate, and NADPH to form glutamate and NADP+, which is detected spectrophotometrically instruments;
- Most common on automated instruments; accurate and precise
A
catalyzed by GLDH
5
Q
ammonia
clinical applications
* Provide useful information on clinical conditions such as (?), (?) and inherited deficiencies of the urea cycle enzymes
- Monitor hyperalimentation therapy
- Measurement of (?) can be used to confirm the ability of the kidneys to produce ammonia
A
- hepatic failure
- reye’s syndrome
- urine ammonia
6
Q
ammonia methods
- Diffusion of NH3 through selective membrane into NH4Cl causes a pH change, which is measured potentiometrically
- Good accuracy and precision; membrane stability may be a problem
A
ion-selective electrode
