Alain Papers

Question 1

Some intro. to the research

Answer 1

1. Fluorescence spec. is highly versatile tool widely applied in biomolecular research.
2. Low intrinsic fluorescence of DNA. –> introduction of fluorescence probes.
3. Fluorophore exhibit extreme sensitivity to envir. changes.
4. 2-amino-purine is an intensiometric probe.
5. Ratiometric sensing (fluctuation in the intensity, concentration of the applied dye)

Question 2

Principal of 3HCs method

Answer 2

1. 2 excited state => provide 2 separates emission bands
2. offer exceptional solvatochromism (^ H-bond donor strength and dielectric constant of solvent =>decrease relative intensity of the T*)
3. Changes in the polarity and the hydration of microenvironment of 3HCs can monitor by measuring the ratio intensity /./ 2 emission band.

Question 3

MCC:Purpose of this paper

Answer 3

to publish a probe (analog of deoxyuridine) for discriminating matched from mismatched duplexes.

Question 4

MCC: Fluorescent nucleosides with high sensitivity to H-bonding are great interested of

Answer 4

1. Investigate DNA hydration
2. DNA protein interactions and dynamics
3. Develop screening assays.

Question 5

MCC: Water coating of DNA is a key role to determine

Answer 5

Its folding, conformations and interactions

Question 6

MCC: the dielectric constant Lippert Mataga scale were employed to estimate

Answer 6

the polarity of the local environment of the probes.

However, in hydrated media is not satisfactory.

Question 7

MCC: Wt’s DAN?

Answer 7

Dansyl fluorophores

Question 8

MCC: Wt need to be measuring?

Answer 8

1. Intensity ratios of the 2 emission band (I_N/I_T)
2. Lambda shifts of the T* band
3. Quantum yield in water.