Alain 4

Question 1

Specific characteristics of suicide substrates

Answer 1

1. Need catalytic activation
2. trapping agents
3. K_4/k_inact

Question 2

Affinity labels are useful for

Answer 2

1. active site mapping - directed mutagenesis.
2. receptor topology (photolabelling)
3. several drugs used in clinics.

Question 3

Intro. penicillins

Answer 3

1. Antibacterial agents - inhibit bacterial cell wall
2. Disc. Fleming, fungal colony, 1928
3. non toxic and antibacterial
4. Puri. Florey and Chain (1938)
5. Clinical trial (1944)
6. X-ray crystallography
7. Syn. Sheehan (1957)
8. 6-APA by Beechams (1958-60)
9. clavulanic acid and B-lactamase inhibitors

Question 4

Wt’s 6-APA?

Answer 4

6-aminopenicillanic acid

This is the pharmacophore of penicillins

Question 5

Mechanism of action (penicillins)

Answer 5

Covalent bond, transpeptidase enzyme + (B-lactam ring)

Irreversible inhibition

Question 6

Wt’s PLP?

Answer 6

Pyridoxal phosphate (vitamin B6)

Question 7

GABA?

Answer 7

Grama-Aminobutyric acid

one of the most important neurotransmitter inhibitor of CNS.

Question 8

For remember 20 aa

Answer 8

Glaciers in Alaska victoriously locate isolated PROwlers
Aroma of Pine, Timber and other Trees
Alcohol is a SERIous TREat!!
Basically, History Love Kind Adventurers Roughly
We Methodically Check the path for SULFUR
Acid, as per knowledge it can’t be Doubled, are a component of GluE.
Amide this Neatly stacked Asparagus is a Glutenous Quail!!!