AGE

Question 1

Steps/Solution for AGE

Answer 1

1X TAE Buffer > EtBr >Loading Dye > Molecular Weight Ladder

Question 2

Components of 1X TAE Buffer

Answer 2

Tris base
Glacial acetic acid
EDTA

Question 3

fairly constant pH and able to conduct electricity because of the concentration of hydrogen ions

Answer 3

TAE Buffer

Question 4

TAE Buffer has a conductivity that allows

Answer 4

fast migration of DNA

Question 5

Components of Ethidium Bromide

Answer 5

3,8-diamino-5-ethyl-6-phenylphenanthridinium

Question 6

used as a non-radioactive marker to stain DNA to visualize nucleic acid bands in electrophoresis

Answer 6

eTbr

Question 7

The mode of binding of EtBr is _______

Answer 7

intercalation between base pairs

Question 8

EtBr’s_____ require special safe handling and disposal

Answer 8

hazardous (mutagen) properties

Question 9

Components of Loading dye

Answer 9

bromophenol blue or xylene cyanol

Question 9

set of standards that are used for determining the approx size of a protein or nucleic acid fragment run on an electrophoresis gel

Answer 9

Molecular Weight Ladder

Question 9

Gel loading dyes serve a few purposes which are

Answer 9

color sample, keep DNA from diffusing, help keep track of migration of sample

Question 9

Markers should be loaded in ____ lanes to samples

Answer 9

adjacent lanes