Adenovirus Flashcards
Adenoviruses are useful for what kind of studies?
Adenoviruses are especially valuable systems for
molecular and biochemical studies of eukaryotic cell processes.
They are also useful vectors for gene therapy approaches.
Features of adenoviruses virion?
Icosahedral, 70–90 nm in diameter, 252 capsomeres; fiber projects from each vertex
Genome of adenoviruses
Double-stranded DNA, linear, 26–45 kbp, protein bound to termini, infectious
Replication site for adenoviruses
Cells of epithelial origin
The replicative cycle is sharply divided into early and late events
Site for adenoviruses replication
Nucleus
Site for adenoviruses uncoating
Begins in the cytoplasm and completed in the nucleus
with release of viral DNA perhaps occurring at the nuclear membrane
When does early event occur?
They are the steps that occur before the onset of viral DNA synthesis
What are the goals of the early event
The goals of the early events are
1) to induce the host cell to enter the S phase of the cell cycle to create conditions conducive to viral replication
2) to express viral functions that protect the infected cell from host defense mechanisms
3) and to synthesize viral gene products needed for viral DNA replication.
The E1A early gene is especially important for?
it must be expressed for the other early regions to be transcribed.
Modulation of the cell cycle is accomplished by the E1A gene products.
The E1B early region is for?
The E1B early region encodes proteins that block cell death (apoptosis) occurring as a result of E1A functions
What protein represents the early protein?
The early proteins are represented by the 75-kDa DNA-binding protein
When does late event begin?
Late events begin concomitantly with the onset of viral DNA synthesis.
Where does protein synthesis for adenoviruses occur?
Cytoplasm
What is the eclipse period?
The time between infection and the first appearance of progeny virus is the eclipse period
