Additional Questions2

Question 1

Non-competitive Inhibitor

Answer 1

• Very rare
• Inhibitor binds to free enzyme and ESC
• Lowers the # of free enzyme molecules
• Vmax reduced, Km not usually effected

Question 2

Lineweaver-Burk Plot equation (LWBP)

Answer 2

• Straight line graph

Question 3

Michaelis-Menten Graph + Equation

Answer 3

V = (Vmax.[S])/(Km + [S])

Question 4

Effect of Competitive inhibitor on LWBP

Answer 4

• Gradient increases
• Y intercept does not change
• X intercept (1/Km) gets smaller (because it is a reciprocal, so the bigger Km gets, the smaller the 1/Km gets)

Question 5

Effect of Uncompetitive inhibitor on LWBP

Answer 5

• Gradient is the same
• Y intercept (1/Vmax) increases
• X intercept (-1/Km) increases

Question 6

Effect of Non-competitive inhibitor on LWBP

Answer 6

• Gradient increases
• Y intercept (1/Vmax) increases
• X intercept does not change