addition q 3 Flashcards
1
Q
Finding Km from the LWBP
A
- x intercept = -1/Km
- -ve reciprocal of this
2
Q
Finding Vmax from the LWBP
A
- y intercept = 1/Vmax
- Reciprocal of this
3
Q
Specificity Constant
A
- Kcat/Km
- Larger is better
4
Q
Complex I
- Coenzyme
- What transfer of electrons takes place
A
- NADH –> NAD
- CoQ –> CoQH2- e- from
5
Q
Complex II
- Coenzymes
- What transfer of electrons takes place
A
- FADH –> FAD
- CoQ –> CoQH2
6
Q
Complex III
- Coenzymes
- What transfer of electrons takes place
A
- CoQH2 –> CoQ
- Cytochrome C –> reduced Cytochrome C
7
Q
Complex IV
- Coenzymes
- What transfer of electrons takes place
A
- reduced Cytochrome C –> Cytochrome C
- 2e- + 2H+ +½O2 –> H2O
8
Q
malate dehydrogenase
A
oxidises malate to produce NADH
9
Q
succinate dehydrogenase
A
oxidises Succinate into fumarate producing FADH
10
Q
P/O ratio
A
nmol O2 added/ mol O2 used
11
Q
To work out P/O from a graph
A
- (mm oxygen used/mm oxygen total) * (whatever nmol O2 100% oxygen saturation was). This works out O2 used- Divide total nmol of oxygen by this number
12
Q
Dinitrophenol
A
- Proton carrier
- Carries the protons from the inner membrane space to inside the matrix, where they can react with oxygen to form water
- This by-passes the proton gradient and ATP, so oxygen consumption occurs much quicker in the presence of dinitrophenol
- It is a weak acid: gets protonated inside the inter-membrane space, and is now lipid soluble. Diffuses across the membrane down the concentration gradient, loses its proton, can no longer cross the mebrane
13
Q
Amytal
A
- Inhibits complex I
- When substrate is malate (so NAD produced), little change in oxygen consumption
14
Q
Antimycin A
A
- Inhibits Coenzyme Q/Complex III or IV- cannot block 2 different active sites, so it must block the Coenzyme Q which is after this
15
Q
Oligomycin
A
- Inhibits ATP synthase
