ADD NOTES

Question 1

What is the SAMPLE & REAGENT used for forward typing?

Answer 1

patient red blood cells + Anti-sera reagents (Anti-A and Anti-B)

Question 2

What is the SAMPLE & REAGENT used for reverse typing?

Answer 2

patient serum + reagent A cells and reagent B cells

Question 3

physical expression of a genotype

Answer 3

PHENOTYPING

Question 4

example AO but phenotypes as Group A

Answer 4

GENOTYPE

Question 5

having 2 identical alleles of a particular gene or

genes

Answer 5

HOMOZYGOUS

Question 6

the presence of 2 different alleles at a particular

gene locus.

Answer 6

HETEROZYGOUS

Question 7

Represents the inheritance of a double dose of the h gene (hh) & its RBCs do not react with the anti-H lectin (Ulex eropaeus)

Answer 7

BOMBAY PHENOTYPE Oh

Question 8

Occurs if the child expressed an antigen that he could not have inherited from
the potential father.

Answer 8

DIRECT EXCLUSION

Question 9

Occurs when a genetic marker is absent in the child but should have been
transmitted by the alleged father

Answer 9

INDIRECT EXCLUSION

Question 10

seen only in Group A individuals.

associated with??

Answer 10

acquired B

colorectal malignancy, GI obstruction, gram-negative
sepsis

Question 11

At what pH does not agglutinate acquired B cells?

Answer 11

pH of 6.0

Question 12

Serves as a source for Anti-A1 reagent. This is used to differentiate A1 from A2 phenotypes

Answer 12

Dolichus biflorus

Question 13

Arrange the Ag from greatest to least?

Answer 13

O > A2 > B > A2B > A1> A1B

Greatest Least

Question 14

Most Rh antibodies are ____ immunoglobulins and react optimally at _____

Answer 14

Ig G, 37C

Question 15

ABO blood group system has naturally occurring antibodies that are
primarily ____

Answer 15

Ig M

Question 16

If C is in trans position

Answer 16

C in Trans to RHD

Question 17

D antigens expressed are complete but fewer in number

Answer 17

Weak D

Question 18

D antigen expression is weakened when one or more D epitopes within the entire D protein is either missing or altered.

Answer 18

Partial D

Question 19

Individuals whose RBC possess an extremely low number of D antigen sites.

Answer 19

Del

Question 20

has anti-P specificity with biphasic activity

Answer 20

Donath-Landsteiner

Question 21

In Donath-Landsteiner the antibody attaches to RBCs at ___ and then causes the red cells to hemolyze
when warmed to ____

Answer 21

4C, 37C

Question 22

contain mostly I antigen, and anti I would react with all _____ found on screen or panels cells.

Answer 22

Adult cells (2)

Question 23

contain mostly i antigen and would test negative or only weakly positive with anti I

Answer 23

Cord cells

Question 24

Compatibility testing may be performed on a patient sample ____ of the scheduled transfusion

Answer 24

w/n 3 days

Question 25

“stacks of coins” appearance of RBCs | CAUSES?

Answer 25

Rouleaux formation | Causes: increased serum proteins like fibrinogen and globulins

Question 26

this term refers to removing or “dissociation”of an antibody that is attached to the surface of an RBC.

Answer 26

Elution

Question 27

adherence of an antibody or antigen onto the surface of an RBC.

Answer 27

Adsorption

Question 28

associated with antibodies to human leukocyte antigens or neutrophil antigens that react with patient granulocytes causing acute respiratory insufficiency.

Answer 28

TRALI

Question 29

Patients with ____ cannot fight bacterial infection because of dysfunctional phagocytic enzymes

Answer 29

CGD

Question 30

Patients with _____ antibodies should not receive components containing plasma.

Answer 30

anti-IgA

Question 31

prospective donor is unable to donate blood for | a limited period of time

Answer 31

Temporary deferral

Question 32

Prospective donor is unable to donate blood for someone else for an unspecified period of time due to current regulatory requirements

Answer 32

Indefinite deferral

Question 33

Prospective donor will never be eligible to | donate blood for someone else.

Answer 33

Permanent deferral

Question 34

enhances the separation of white cells from the red cells

Answer 34

Hydroxyethyl starch