Adaptameres

Question 1

What are Adaptermeres

Answer 1

ssRNA;ssDNA (or small peptide) that forms 3D structure capable of specific molecular recognition against peptides, metal ions etc.

Question 2

Difference between RNA and DNA adaptameres

Answer 2

RNA:
- more diverse and interactive structures
- Can form smaller structures with same amount of nucleotides
- More expensive to produce
DNA:
- More stable but RNA can be stabelized

Question 3

SELEX

Answer 3

Systematic Evolution of Ligands by Exponential enrichment
–> Varable region in the middle of the strain –> Combinational Library
- Innitial Library is is added to the purifyed protein
- Removal of unbound adaptameres and recovery of bound, 2-15 reps
- Reverse transcriprion and amplification
- Sequencing to ID adaptamere

Question 4

What makes adaptameres special (Compared to Ab)

Answer 4

• Smaller
• Reversal of activity with complimentary strain
• Sees smaller targets –> point mutations and Isomeres also inacessable (to Ab) domains
• Lower Imunogenicity
• Faster and easyer to produce
• Short circulation time
• Smaller therapeutic effect (VEGF)

Question 5

Polyanionic effect

Answer 5

adaptameres are highly negatively charged and bind blood protein –> high uptake in non targets
To circumvent do SLEX in presence of competing blood protein