Acute Inflammation

Question 1

Which molecule is NOT found in the lateral border recycling compartment (LBRC) of endothelial cells?

a. VE-cadherin
b. CD99
c. PECAM
d. JAM-A
e. JAM-C

Answer 1

a, Getting Leukocytes to the Site of Inflammation. Muller WA. Vet Path. 2013. 50:7-22. Page 11

Question 2

Exposure of bovine adherent peripheral blood mononuclear cells leads to E. coli O157:H7 verotoxin has been shown to increase cellular level of all of the following EXCEPT

a. phospho-p38 MAP kinase
b. phospho-ERK
c. phospho- IK
d. TNF- secretion
e. All of the above are increased with exposure to verotoxin

Answer 2

c, J Comp Path 2012. Vol 147: 20-23. Verotoxin 2 activates mitogen-activated protein kinases in bovine adherent peripheral blood mononuclear cells

Question 3

All of the following is true regarding the loosening of cellular tight junctions during leukocyte diapedesis EXCEPT

a. Clustering of ICAM-1 mediated by LFA-1 binding leads to activation of RhoA
b. ICAM-1 stimulation leads to dephosphorylation of VE-cadherin to destabilize VE-cadherin interactions with the cytoskeleton
c. VCAM-1 cross linking activates Rac-1 and increases reactive oxygen species
d. VCAM-1 cross linking leads to an increase in cytosolic calcium
e. Transendothelial migration does not absolutely require the loosening of tight junctions

Answer 3

c, Robbins and Cotran, 6th Ed 2010 p120 ICAM-1 stimulation phosphorylates VE-cadherin. Note that transendothelial migration may also proceed transcellularly or through the lateral border recycling compartment.

Question 4

In the lymph node, HEVs constitutively produce all of the following EXCEPT:

a. CCL19
b. CCL21
c. CXCL12
d. CXCL13
e. CCR7

Answer 4

e; PBVD 5th ed, p. 134

Question 5

. Which of the following is activated by anthrax lethal toxin?

a. NLRP1 inflammasome
b. NLRP3 inflammasome
c. NLRC4/IPAF inflammasome
d. AIM2 inflammasome
e. RIG-1

Answer 5

a, Cell 2010, 140, 821-832. The inflammasomes

Question 6

Which of the following is NOT true about NK cells?

a. IL-21 regulates differentiation and apoptotic death induced by NK cells.
b. Type II NK cells express CD56.
c. CD16 is necessary for antibody dependent cellular cytotoxicity mediated by NK cells.
d. NK-T lymphocytes recognized CD1d antigen presenting molecule and on activation secrete IFN, IL-4, GM-CSF.
e. NK cells kill target cells through release of perforins from cytoplasmic granules.

Answer 6

b. PBVD 5th edition. p: 102

Question 7

What two cell types produce gelatinase (MMP-2)?

a. Fibroblasts and pericytes
b. Macrophages and neutrophils
c. Macrophages and fibroblasts
d. Endothelial cells and pericytes
e. Endothelial cells and fibroblasts

Answer 7

e, PBVD, pg. 140