Activity 12 (FACTORS AFFECTING ENZYME ACTIVITY) Flashcards
Factors affecting enzyme activity: (5)
SUBSTRATE CONCENTRATION,
ENZYME CONCENTRATION,
TEMPERATURE,
pH OF THE RXN,
PRESENCE OF ACTIVATORS OR INHIBITORS
A rise in __ increases the __ of an enzyme rxn within a narrow temperature range.
TEMPERATURE, VELOCITY
An enzyme rxn’s velocity is doubled for a?
10-degree rise in temperature.
If the temperature increases beyond __ , the rxn rate ___.
60°C, DECREASES
This destroys enzyme activity
BOILING
Optimum temperature of enzyme in body fluids
37°C
Since enzymes are proteins, they are sensitive to?
pH CHANGES
A change in pH may alter the ionic character of the ___ and ___ groups on the protein surface of the enzyme.
AMINO, CARBOXYLIC
When amino and carboxylic groups are altered on the protein surface of the enzyme, these changes will affect the?
SHAPE AND CATALYTIC ACTIVITY OF THE ENZYME.
This is called the pH at which the rxn rate is at maximum.
OPTIMUM PH
Enzyme action is usually demonstrated by:
PRODUCT’S INCREASED FORMATION AND
THE SUBSTRATE’S DECREASED CONCENTRATION
Some enzymes are active as?
PURE PROTEIN
This is required as an additional component for other enzymes to be catalytically active
COFACTORS
3 types of cofactors
PROSTHETIC GROUPS,
COENZYMES,
METAL ACTIVATORS
These are tightly bound to the enzyme proteins
PROSTHETIC GROUPS
