Activity 11 (ENZYMES) Flashcards
Principle of enzymes:
ALL ENZYMES ARE PROTEINS BUT NOT ALL PROTEINS ARE ENZYMES
It is a protein synthesized in the living cells, which catalyzes THERMODYNAMICALLY possible rxns so that the rxn rate is compatible with the biochemical processes for maintaining the cell.
ENZYME
Short definition for enzyme
ENZYMES ARE BIOLOGICAL CATALYSTS
Some enzymes consist ___ __ ___, while others contain __ __, and others are similar to __ __.
WHOLLY OF PROTEINS
METALLIC IONS
CONJUGATED PROTEINS
This is what we called for the protein portion.
APOENZYME
This is what we called for the nonprotein organic group.
COENZYME
APOENZYME + COENZYME = ?
HOLOENZYME
This is the active enzyme when apoenzyme and coenzyme form together.
HOLOENZYME
The specificity of action is the major difference between an inorganic catalyst, such as __ and __ , and __ .
NICKEL AND PLATINUM
ENZYME
Enzymes act only on particular substances. The substance upon which an enzyme works is called?
SUBSTRATE
Different enzymes exhibit __ , ___ , __ , and ____ ___.
ABSOLUTE
STEREOCHEMICAL
LINKAGE
GROUP SPECIFICITIES
The high specificity of an enzyme is due to its?
PROTEIN NATURE
The region of the protein portion where the substrate binds.
ACTIVE SITE
The active site on the enzyme’s surface is so contoured and charged that it attracts only the substrate to form an?
ENZYME SUBSTRATE COMPLEX
PROCEDURE FOR ACTIVITY 11
A. PREPARATION OF CATALASE
1. BIURET TEST
2. TEST FOR CATALASE ACTIVITY
3. PREPARATION OF A DILUTE SOLUTION OF SALIVARY AMYLASE