Act 6

Question 1

it is the technique of separation and identification of chemical substances by moving solvent (mobile phase) in a thin layer of sultable adsorbent (stationary phase) with a binder serving as inert support for the layer

Answer 1

thin layee chromatography

Question 2

the mechanism of separation involved is called?

Answer 2

adsorption

Question 3

both sample and solvent molecules may adhere to the surface of the adsorbent through?

Answer 3

weak intermolecular forces

Question 4

what is the reversed equilibrium?

Answer 4

X(mobile) + nS(stationary) - X(stationary) + nS(mobile)

Question 5

x is the?
s is the?

Answer 5

sample
solvent

Question 6

it is applicable for the separation of several compounds but is more satisfactory for non-polar substances

Answer 6

adsorptive chromatography

Question 7

this compound will not interact strongly with a polar phase

Answer 7

non polar compound

Question 8

this will not interact strongly with non polar phase

Answer 8

polar compound

Question 9

it is a sheet of glass, metal, or plastic (polyethylene) which is coated with a thin layer of solid adsorbent, usually silica or alumina

Answer 9

TLC plate

Question 10

If too much sample is spotted, some compounds _____________

Answer 10

will not be retained because it cannot interact with stationary phase

Question 11

PROCEDURE ACT 6 A.) about preparation of analgesic sample solution

Answer 11

1. Grind
2. Add 5ml
3. Repeat step 1 & 2

Question 12

procedure B. preparation of TLC plate for the analgesic sample solution

Answer 12

1. Gently draw
2. draw pencil dots
3. make several micropipettes
4. Dip
5. Gently dab the end
6. repeat procedure

Question 13

procedure C. developing TLC plate

Answer 13

1. carefully put 2 ml
2. using forceps
3. cover chamber, carefully place
4. using forceps, carefully remove
5. visualize
6. measure distance
7. calculate rf
8. submit