Act 5

Question 1

It is the physical separation of components of a mixture on the basis of differing affinities for stationary vs mobile phase

Answer 1

Chromatography

Question 2

Chromatography is the most common ______ and ______ separation techniques

Answer 2

chemical and biochemical

Question 3

this technique is valuable for qualitative and quantitative monitoring of reactions

Answer 3

chromatography

Question 4

when the stationary phase is planar, such as paper/coated glass plate, it is called as?

Answer 4

planar chromatography

Question 5

why do paper chromatography works?

Answer 5

because of the “capillary action” of water, or solvent in paper

Question 6

this allows us to separate compounds due to attraction/affinity of compounds for the mobile phase (solvent) vs. stationary phase (paper/thin film)

Answer 6

capillary action

Question 7

• Compounds that are NOT soluble in solvent, very attracted to stationary phase, _________
• Compounds that are VERY soluble in solvent, less attracted to stationary phase, _______

Answer 7

• stays in place
• travel up to stationary phase with solvent

Question 8

K is the?

Answer 8

partition coefficient

Question 9

Rf (retention factor) = ?

Answer 9

distance travelled by the substance/ distance travelled by the solvent

Question 10

the relative distance of travel of the substance is?

Answer 10

retention factor (rf)

Question 11

how many ml of solvent should be poured in the developing chamber?

Answer 11

20 ml