ACP Flashcards
Hydrolase that catalyzes hydrolysis of various phosphomonoesters at an acidic pH (5.0)
Acid Phosphatase
Acid Phosphatase catalyzes hydrolysis of various phosphomonoesters at what pH
an acidic pH (5.0)
richest tissue source
Prostate
what are the tissue sources
- Prostate (richest source)
- Bone
- Liver
- Spleen
- Kidney
- RBC
- Platelets
clinically significant in the diagnosis of ___
prostatic carcinoma
in forensic medicine, this marker is also relevant in ___
rape cases
diagnostic significances involves
Prostatic carcinoma
* Investigation of rape cases
* Bone diseases like
Paget’s disease and Gaucher’s disease
* Platelet damage
Gaucher cells are what type of cell
scavenger cells
inhibited by tartrate
Prostatic ACP
not inhibited by tartrate
Non prostatic ACP
this results to Total ACP Activity
(prostatic ACP + non prostatic ACP)
Activity of ACP on a serum without tartrate
Two sample preparation in ACP Isoenzyme
- Activity of ACP on a serum without tartrate
- Activity of ACP on a serum with tartrate
this results to non prostatic ACP activity only
Activity of ACP on a serum with tartrate
Substrates
orthophosphoric monoesters
Bodansky Method
B-glycerophosphate or Na- glycerophosphate
King-Armstrong or Gutman-Gutman Method
phenylphosphate or disodium phenylphosphate
Babson and Reed Method
α-1-naphthyl
phosphate as substrate
substrate of choice for quantitative end point reactions
Thymolphthalein monophosphate
Immunochemical techniques for prostatic ACP
- RIA,
counterimmunoelectrophoresis,
and immunoprecipitation. - Immunoenzymatic assay (Tandem
E)
