ACh receptors (Week 7)

Question 1

what are some advantages of cell attached clamp

Answer 1

1)can record the activity of single receptors
2)the intracellular milieu is marinated (no wash-out of intracellular biochemistry)
3)Control of extracellular solution

Question 2

What are some disadvantages of cell-attached patch clamp

Answer 2

1) investigating different concentrations of drugs
e.g. agonist is complex
2) the agonist always presents - issue with receptor desensitization
3) no control of the intracellular
4) resting membrane potential is unknown

Question 3

what are some advantages of whole cell voltage clamp

Answer 3

• control of the intracellular and extracellular milieu
• can record from population of receptors/ ion channels
• can introduce biochemical modulators (intracellularly) via the recording pipette

Question 4

what are some disadvantages of whole-cell voltage clamp

Answer 4

• biochemical “washout” of the intracellular milieu
• Resolution - can rarely observe single receptor activity

Question 5

what are some advantages of outside-out patch

Answer 5

-control of the intracellular and extracellular milieu
- can change/control extracellular drug (agonist) concentrations

Question 6

what are some disadvantages of outside out patch

Answer 6

-biochemical washout of the intracellular milieu
-cannot easily change intracellular messengers e.g. c-AMP

Question 7

application of whole cell patch clamp

Answer 7

forms tight seal between glass pipette and the cell membrane, allowing the researcher to have control over the intracellular environment

Question 8

use of the whole cell patch clamp

Answer 8

to study overall membrane conductance, current voltage relationships and whole cell responses to various stimulus. suitable for studying voltage gated ion channels and ionotropic receptors.

Question 9

applications of cell-attached patch clamp

Answer 9

glass pipette sealed onto cell membrane without distrupting it

Question 10

use of cell attached patch clamp

Answer 10

to study activity of ion channels in cellular environment. researchers able to observe spontaneous open and closing of ion channels and understand behaviour

Question 11

application of inside-out patch clamp

Answer 11

cell membrane excised, exposing intracellular side of membrane

Question 12

use of inside out patch clamp

Answer 12

for studying regulation of ion channels by intracellular factors. allows researchers to manipulate the composition of the intracellular solution and study the effects on channel activity.

Question 13

outside-out patch clamp application

Answer 13

patch with ion channels is excused with the extracellular side facing the recording solution

Question 14

outside-out patch clamp application

Answer 14

useful for studying effects of extracellular factors on ion channels. Researchers can manipulate composition of the extracellular solution and observe the impact on channel activity.