AA Sequence: Qualitative Test

Question 1

Ninhydrin

Answer 1

RG: alpha amino acids
Result: Ruhemann’s purple

Question 2

RG: alpha amino acids
Result: Ruhemann’s purple

Answer 2

Ninhydrin

Question 3

Aldehydes

Answer 3

RG: NH2 of Lysine
Result: Schiff’s base adducts

Question 4

RG: NH2 of Lysine
Result: Schiff’s base adducts

Answer 4

Aldehydes

Question 5

Sakaguchi Test

Answer 5

RG: guanidine group of Arginine
Result: red-orange complex

Question 6

RG: guanidine group of Arginine
Result: red-orange complex

Answer 6

Sakaguchi Test

Question 7

Xanthoproteic Test

Answer 7

RG: Substituted benzene
Result: yellow precipitate/solution

Question 8

RG: Substituted benzene
Result: yellow precipitate/solution

Answer 8

Xanthoproteic Test

Question 9

PB(OAc)2

Answer 9

RG: -SH of Cysteine
Result: black precipitate, PbS

Question 10

RG: -SH of Cysteine
Result: black precipitate, PbS

Answer 10

PB(OAc)2

Question 11

Dithionitrobenzoic acid

Answer 11

RG: -SH of Cysteine
Result: yellow product

Question 12

RG: -SH of Cysteine
Result: yellow product

Answer 12

Dithionitrobenzoic acid

Question 13

Pauly Reagent

Answer 13

RG: imidazole of Histidine, and phenol of Tyrosine
Result: reddish or yellow product