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Chem 41 > AA Sequence: Chemical/Enzymatic Analysis > Flashcards

AA Sequence: Chemical/Enzymatic Analysis Flashcards

1
Q

DNFB/Sanger Reagent

A

Determine N-terminal AA producing a dinitrophenol (DNP)-AA

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2
Q

Determine N-terminal AA producing a dinitrophenol AA

A

DNFB/Sanger Reagent

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3
Q

Phenylisothiocyanate / Edman Reagent

A

Determine N-terminal AA producing a phenylthiohydantoin (PTH) - AA and cleaving this from the parent peptide

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4
Q

Hydrazine

A

determine C-terminal AA

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5
Q

Determine N-terminal AA producing a phenylthiohydantoin (PTH) - AA and cleaving this from the parent peptide

A

Phenylisothiocyanate / Edman Reagent

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6
Q

determine C-terminal AA

A

Hydrazine

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7
Q

Cyanogen bromide

A

cleaves peptide bond wherein carboxyl groups are contributed by the AA;

Methionine

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8
Q

cleaves peptide bond wherein carboxyl groups are contributed by the AA;

Methionine

A

Cyanogen bromide

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9
Q

Hydroxylamine HCl

A

cleaves peptide bonds joining asparagine and glycine

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10
Q

cleaves peptide bonds joining asparagine and glycine

A

Hydroxylamine HCl

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11
Q

Iodosobenzoate

A

cleaves at carboxyl side of tryptophan residues

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12
Q

cleaves at carboxyl side of tryptophan residues

A

Iodosobenzoate

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13
Q

2-nitro-5-thiocyanobenzoate

A

cleaves at amino side of cysteine

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14
Q

cleaves at amino side of cysteine

A

2-nitro-5-thiocyanobenzoate

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15
Q

Trypsin

A

cleaves at peptide bonds wherein carbonyl group is contributed by BASIC residues, lysine and arginine

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16
Q

cleaves at peptide bonds wherein carbonyl group is contributed by BASIC residues, lysine and arginine

17
Q

Chymotrypsin

A

cleaves at carboxyl side of AROMATIC amino acids: tyrosine, phenylalanine, tryptophan

18
Q

cleaves at carboxyl side of AROMATIC amino acids: tyrosine, phenylalanine, tryptophan

A

Chymotrypsin

19
Q

Carboxypeptidase A

A

cleaves all C-terminal except proline

20
Q

cleaves all C-terminal except proline

A

Carboxypeptidase A

21
Q

Carboxypeptidase B

A

cleaves C-terminal amino acids if lysine and arginine only

22
Q

cleaves C-terminal amino acids if lysine and arginine only

A

Carboxypeptidase B

23
Q

Thermolysin

A

cleaves amino side of residues where R is contributed by leucine and valine

24
Q

cleaves amino side of residues where R is contributed by leucine and valine

A

Thermolysin

25
Q

Streptococcal protease

A

cleaves at carboxyl side of ACIDIC amino acids

26
Q

cleaves at carboxyl side of ACIDIC amino acids

A

Streptococcal protease

27
Q

Thrombin

A

cleaves at carboxyl side of arginine

28
Q

cleaves at carboxyl side of arginine

29
Q

Elastase

A

cleaves at carboxyl side of alanine

30
Q

cleaves at carboxyl side of alanine

Chem 41 (2 decks)

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