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CLINICAL CHEM PRACTICE Q'S > AA & Protein > Flashcards

AA & Protein Flashcards

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1
Q

how do you distinguish between two aa

A

r-group

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2
Q

what is the charge of an aa when the pH is less than pH of isoelectric point

A

cation

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3
Q

what is the charge of an aa when the pH is more than pH of isoelectric point

A

anion

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4
Q

what is the charge of an aa at the isoelectric point

A

electrically neutral

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5
Q

formula for pH

A

= pk1+pk2/2

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6
Q

what is the aminoacidopathy of a PAH enzyme defect

A

PKU

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7
Q

what is the aminoacidopathy of a FAAH enzyme defect

A

Tyrosinemia I

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8
Q

what is the aminoacidopathy of a TAT enzyme defect

A

Tyrosinemia II

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9
Q

what is the aminoacidopathy of a 4-HPPO enzyme defect

A

tyrosinemia III

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10
Q

what is the aminoacidopathy of a HGO enzyme defect

A

alkaptonuria

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11
Q

if a pt has PKU what protein conc goes up

A

phenylalanine

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12
Q

the Guthrie screening test for increased serum phenylalanine is based on

A

the microbiologic procedure in which phenylalanine counteracts the effects of metabolic antagonist on the growth of B. subtilis

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13
Q

what sets proteins apart from pure carbohydrates and lipids

A

nitrogen content

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14
Q

when a protein is dissolved in a buffer soln the pH of which is more alkaline than the pI, and an electric current is passed through the soln the protein will act as

A

an anion and migrate to the anode

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15
Q

when you drop a peptide chain in water what will happen to the polypeptide chain

A

folds

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16
Q

what is the driving force for protein folding

A

hydrophobic and hydrophilic

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17
Q

what are the forces holding a protein in its 3D shape

A

all chem forces

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18
Q

the 3D spatial configuration of a single polypeptide chain as determined by disulfide linkages, H-bonds, electrostatic attractions, and van Der walls forces is referred to as the

A

tertiary structure

19
Q

the 3D spatial configuration of two polypeptide chain as determined by disulfide linkages, H-bonds, electrostatic attractions, and van Der walls forces is referred to as the

A

quaternary structure

20
Q

which of the following is not a protein function:
a. serves as a structure component
b. catalyze biochem rxns such as enzymes
c. possess genetic code such as DNA

A

C

21
Q

the plasma protein mainly responsible for maintaining colloidal osmotic pressure in vivo is

A

albumin

22
Q

what is the function of transferrin

A

transports iron

23
Q

what is the function of haptoglobin

A

binds free hemoglobin

24
Q

what is the function of a-antitrypsin

A

inhibits proteolytic enzymes

25
Q

the following pattern of serum protein electrophoresis is obtained:
Albumin- decreased
alpha 2 globulin- increased
gamma globulin- normal

A

acute inflammation

26
Q

does albumin increase or decrease in hypoalbuminemia

A

decrease

27
Q

does alpha 1 globulin increase or decrease in hypoalbuminemia

A

increase

28
Q

does gamma globulin increase or decrease in hypoalbuminemia

A

increase

29
Q

does albumin increase or decrease in immediate response

A

decrease

30
Q

does alpha 2 globulin increase or decrease in immediate response

A

increase

31
Q

does albumin increase or decrease in delayed response pattern

A

decrease

32
Q

does alpha 2 globulin increase or decrease in delayed response pattern

A

increase

33
Q

does gamma globulin increase or decrease in delayed response pattern

A

increase

34
Q

does albumin increase or decrease in nephrotic syndrome

A

decrease

35
Q

does alpha 2 globulin increase or decrease in nephrotic syndrome

A

increase

36
Q

does gamma globulin increase or decrease in nephrotic syndrome

A

decrease

37
Q

does gamma globulin increase or decrease in hypogammaglobulinemia

A

decrease

38
Q

does albumin increase or decrease in hepatic cirrhosis

A

decrease

39
Q

does beta 2 globulin increase or decrease in hepatic cirrhosis

A

increase

40
Q

does gamma globulin increase or decrease in hepatic cirrhosis

A

increase

41
Q

what condition forms bridging between beta 2 globulin and gamma globulin

A

hepatic cirrhosis

42
Q

does albumin increase or decrease in monoclonal gammopathy

A

decrease

43
Q

does gamma globulin increase or decrease in monoclonal gammopathy

A

increase

CLINICAL CHEM PRACTICE Q'S (6 decks)

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