AA & Protein Flashcards
how do you distinguish between two aa
r-group
what is the charge of an aa when the pH is less than pH of isoelectric point
cation
what is the charge of an aa when the pH is more than pH of isoelectric point
anion
what is the charge of an aa at the isoelectric point
electrically neutral
formula for pH
= pk1+pk2/2
what is the aminoacidopathy of a PAH enzyme defect
PKU
what is the aminoacidopathy of a FAAH enzyme defect
Tyrosinemia I
what is the aminoacidopathy of a TAT enzyme defect
Tyrosinemia II
what is the aminoacidopathy of a 4-HPPO enzyme defect
tyrosinemia III
what is the aminoacidopathy of a HGO enzyme defect
alkaptonuria
if a pt has PKU what protein conc goes up
phenylalanine
the Guthrie screening test for increased serum phenylalanine is based on
the microbiologic procedure in which phenylalanine counteracts the effects of metabolic antagonist on the growth of B. subtilis
what sets proteins apart from pure carbohydrates and lipids
nitrogen content
when a protein is dissolved in a buffer soln the pH of which is more alkaline than the pI, and an electric current is passed through the soln the protein will act as
an anion and migrate to the anode
when you drop a peptide chain in water what will happen to the polypeptide chain
folds
what is the driving force for protein folding
hydrophobic and hydrophilic
what are the forces holding a protein in its 3D shape
all chem forces
the 3D spatial configuration of a single polypeptide chain as determined by disulfide linkages, H-bonds, electrostatic attractions, and van Der walls forces is referred to as the
tertiary structure
the 3D spatial configuration of two polypeptide chain as determined by disulfide linkages, H-bonds, electrostatic attractions, and van Der walls forces is referred to as the
quaternary structure
which of the following is not a protein function:
a. serves as a structure component
b. catalyze biochem rxns such as enzymes
c. possess genetic code such as DNA
C
the plasma protein mainly responsible for maintaining colloidal osmotic pressure in vivo is
albumin
what is the function of transferrin
transports iron
what is the function of haptoglobin
binds free hemoglobin
what is the function of a-antitrypsin
inhibits proteolytic enzymes
