A10 Stability of Proteins

Question 1

Uses of precipitation (2)

Answer 1

Concentrates the protein

Purify it with every concentration

Question 2

Heat Denaturation effects on

Casein Albumin Gelatin peptone:

Answer 2

+ + - -

Question 3

HOAc Heat Denaturation effects on

Casein Albumin Gelatin peptone:

Answer 3

+ + - -

Question 4

Isoelectric Precipitation effects on

Casein Albumin Gelatin peptone:

Answer 4

+ - - -

Question 5

Concentrated mineral acids HNO3 effects on

Casein Albumin Gelatin peptone:

Answer 5

+ + - -

Question 6

Organic solvents HOAc 95% alcohol effects on

Casein Albumin Gelatin peptone:

Answer 6

+ + + -

Question 7

Salts of Heavy metals HgCl2 effects on

Casein Albumin Gelatin peptone:

Answer 7

+ + - -

Question 8

Salts of heavy metals CUSO4 effects on

Casein Albumin Gelatin peptone:

Answer 8

+ + - -

Question 9

Salts of Heavy metals FeCl3 effects on

Casein Albumin Gelatin peptone:

Answer 9

+ + - +

Question 10

Alkaloidal reagents Picric acid effects on

Casein Albumin Gelatin peptone:

Answer 10

+ + + -

Question 11

Alkaloidal reagents TCA effects on

Casein Albumin Gelatin peptone:

Answer 11

+ + - -

Question 12

Alkaloidal reagents HOAc, K4Fe(CN)6 effects on

Casein Albumin Gelatin peptone:

Answer 12

+ + - -

Question 13

Salting Out effects on

Casein Albumin Gelatin peptone:

Answer 13

+ + + -

Question 14

Largely responsible for many physical, chemical, and functional properties of proteins

Answer 14

peptide chain conformations

Question 15

Causes denaturation physical:

Answer 15

Heat, violent shaking, high pressures, UV radiation