9 theme: gene expression. Biotechnology

Question 1

Why transfection is performed?

Answer 1

to introduce foreign genetic material into cells to manipulate or study their gene expression.

Question 2

What doe we use to precipitate DNA, that it could be introduced to cell? (transfection)

Answer 2

we use Calcium Phosphate

Question 3

What is DEAE Dextran and what is it used for?

Answer 3

polysaccharide, reagent used for DNA binding

Question 4

What is lipofection used for?

Answer 4

To promote gene expression by delivering DNA into cells via lipid-mediated mechanisms.

Question 5

How microinjection work?

Answer 5

injecting DNA with micropipette straight into nucleus or cytoplasm

Question 6

What is infection?

Answer 6

introduction of genetic material into host cells using viruses

Question 7

When Tetracycline Repressor binds to the Tet operator sites, it blocks transcription.
Is it true?

Answer 7

yes

Question 8

During Tet-On System gene expression …

Answer 8

activated

Question 9

During Tet-of system gene expression is…

Answer 9

expressed

Question 10

How baculovirus work?Why its better than bacteria virus?

Answer 10

Baculoviruses naturally infect insect cells; The gene of interest (the gene that encodes the protein you want to express) is inserted into the baculovirus genome. high yields of recombinant protein

Question 11

What are selection markers used for?

Answer 11

These markers enable researchers to distinguish between cells that have been successfully transformed (or transfected) and those that have not.

Question 12

How does a possitive selective marker show that cell successfully incorporated the desired genetic material?

Answer 12

These markers typically confer resistance to a selective agent (e.g., antibiotic) or enable cells to grow under specific conditions.