9 Flashcards

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1
Q

What is rBGH?

A

(Recombinant bovine growth hormone) a protein that is made by genetically engineered bacteria. It is injected into cows to stimulate a growth hormone

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2
Q

What does a gene (or DNA) encode for?

A

A sequence of DNA that encodes a protein

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3
Q

What is protein synthesis?

A

The process of using instructions carried on a gene to create proteins

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4
Q

What steps are involved in protein synthesis?

A

DNA -transcription- RNA -translation- Gene- protein

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5
Q

What is a protein?

A

A large molecule composed of amino acids

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6
Q

What is DNA?

A

Double-stranded

Each nucleotide is composed of deoxyribose, phosphate, and a nitrogenous base

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7
Q

How many bases does DNA contain?

A

4 bases: adenine (A), thymine (T), guanine (G), and cytosine (C)

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8
Q

What is RNA?

A

Single-stranded with nucleotides composed of A,C,G, and Uracil (U)

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9
Q

Can DNA travel?

A

No, it is stuck in the nucleus

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10
Q

How are DNA and RNA similar?

A

DNA and RNA are both polymers of nucleotides

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11
Q

How are DNA and RNA different?

A

DNA is double stranded and RNA is single stranded

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12
Q

What leaves the nucleus?

A

RNA

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13
Q

What is transcription?

A

The production of an (mRNA) RNA copy of the protein coding DNA gene sequence

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14
Q

Where does transcription occur?

A

In the nucleus

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15
Q

What is translation?

A

Process by which an mRNA sequence is used to produce a protein

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16
Q

Where does translation occur?

A

Outside of the nucleus in the cytoplasm

17
Q

How is the copy of DNA synthesized?

A

By an enzyme called RNA polymerase

18
Q

What does RNA polymerase bind to?

A

It binds to the promoter region of the gene

19
Q

What kinds of RNA are involved in translation?

A

mRNA, tRNA, and rRNA

20
Q

What is the function of mRNA?

A

Carries the genetic information copied from the DNA

21
Q

What is the function of tRNA?

A

Carries amino acids and matches it’s anticodon with codons on mRNA

22
Q

What is made of rRNA and protein?

A

Ribosomes

23
Q

What is a codon and where is it found?

A

A triplet of mRNA nucleotides

24
Q

What is an anticodon and where is it found?

A

A region of tRNA that binds to an mRNA codon

25
Q

Is the sequence ATGCATGC made up of DNA or RNA?

A

DNA

26
Q

What is the complementary DNA sequence for ATGCATGC?

A

TACGTACG

27
Q

What is the complimentary RNA for ATGCATGC?

A

UACGUACG

28
Q

What is a codon compromised of?

A

3 nucleotides

29
Q

What is a mutation?

A

A change in genetic sequence

30
Q

What might affect the order of amino acids in a protein?

A

Mutations

31
Q

What are the possible outcomes of mutation?

A

1 - no chance in protein (neutral mutation)
2 - non-functional protein
3 - different protein

32
Q

What is a base-substitution mutation?

A

A simple substitution of one base for another

33
Q

What is Frameshift mutation?

A

Addition or deletion of a base, which changes the reading frame

34
Q

What sequence stops protein production?

A

UGA, UAA, and UAG (Stop codon)

35
Q

What is a restriction enzyme?

A

Used by bacteria as a form of defense. Cuts DNA at a specific sequence. Allowing scientists to make precise cuts in DNA

36
Q

What is a plasmid?

A

A small, circular piece of bacterial DNA that exists separate from the bacterial chromosome.
Can also act as a ferry to carry a gene into a cell

37
Q

What is a GMO?

A

Genetically modified organisms

The result of the incorporation of a gene from one organism to the genome of another