8.3 -> 8.5 Flashcards
genome
complete set of genes in a cell, including those in mitochondria and chloroplasts
preteome
full range of proteins produced by genome
RNA nucleotide structure
pentose sugar base
one of organic base adenine, guanine, cytosine and uracil
phosphate group
messanger rna
- consists of thosuands of mononucleotides - long strand
- single heix
- posseses info in codons
codon
3 bases that are complementary to triplet in DNA
trasnfer RNA
- small molecules made up of 80 nucleotides
- single-stranded chain folded into clover-leaf shape
- contains binding site for amino acid
- contains anticodon that is specific to amino acid
transcription
- enzyme acts on specific region of DNA, causing two strands to separate and expose nucleotide bases
- nucleotide bases acts as template and pair with complementary nucleotides from the pool
- Enzyme RNA polymerase moves along strand and joins nucleotides together to form pre MRNA molecules(stops when reaches terminator sequence)
- DNA strands rejoin behind rna polymerase
splicing
base sequences corresponding to introns are removed from pre mRNA and functional exons are joined together to from mRNA
stage between transcription and translation
leave nucleus via nuclear pore(too large to diffuse out)
mRNA is attracted to ribosomes to which it becomes attached
intron
a section of DNA that doesn’t code for proteins and interrupts the sequence of genes
exon
Triplet sequence that codes for sequence of amino acids
synthesising a polypeptide(translation)
- ribosome becomes attached to starting codon at one end of mRNA molecule
- tRNA molecule with complementary anticodon sequence(and specific amino acid) moves to ribosome and pairs with codon on mRNA. another tRNA molecules with complementary anticodon pairs with codon on next mRNA
- ribosome moves along mRNA, bringing together two tRNA molecules at any time
- the amino acids on the tRNA are joined by peptide bonds using an enzyme and ATP(hydrolysed)
- tRNA is released from amino acid and is free to collect another specific amino acid
- this process continues until ribosome reaches a stop codon, so mRNA and last tRNA separate and polypeptide chain is complete.