7 DNA

Question 1

restriction enzymes

Answer 1

bind to and cut specific DNA sequences at the restriction sites

Question 2

DNA plasmids

Answer 2

• are used to create recombinant DNA
• contain gene coding regions for proteins and multiple cloning sites
• plasmid DNA is cut at certain restriction site then new DNA is inserted

Question 3

DNA electrophoresis

Answer 3

is used to separate DNA fragments based on size, weight, charge

Question 4

DNA profiling

Answer 4

uses short tandem repeats (STRs) to distinguish unique genetic markers.

Question 5

genetic engineering

Answer 5

DNA molecules can be manipulated/modified through technology

Question 6

complimentary base pairing

Answer 6

adenine-thymine (2 H bonds)
cytosine-guanine (3 H bonds)

Question 7

purine

Answer 7

A & G are double ringed structures

Question 8

pyrimidine

Answer 8

C & T are single ring structures

Question 9

restriction endonucleases

Answer 9

cleaves double stranded DNA which results in sticky or blunt ends

Question 10

EcoRI

Answer 10

it recognizes the sequence GAATTC and cleaves b/w the G & A and creates sticky site

Question 11

EcoRV

Answer 11

it recognizes GATAT and cuts b/w T and A which creates blunt ends

Question 12

recombinant DNA

Answer 12

DNA that is combined from 2 different sources

Question 13

transgenic organisms

Answer 13

have been genetically altered through genetic engineering

Question 14

genetically modified organisms

Answer 14

are transgenic plants