6A Flashcards
Cultivation indépendant methods
DNA can be amplified and sequenced by PCR
Special slide with etched grid
Direct count
Microbial constorita
some microbes are too accostumed to living with neihbors
FISH
Flourescent In Stitu Hybridization
Known volume is added onto the grid and counted with a light microscope
direct count
problem if microbe cannot be cultured
Viable count
Dilutions are made and added to a suitable media and turbidity yeilds MPN
viable count
lag phase
cells synthesizing new components
replenish, adaption, various length
lag phase
cheap/fast/easy
cannot differentiate between living and non living cells
direct count
log/exponential phase
rate of growth and cell division is constant and maximal
Stationary phase
active cells stop producing, or the rate of cells being made and cells dying is equal.
stationary phase is due to:
Nutrient limitation, limited oxygen, waste, and a max population reached
generation time
time to double population
population is most uniform
log/exponential phase
growth rate
number of generations/ time
growth yeild
maximum population density and/or amount of cellular material produced by culture
continuous culture
continual provision of nutrients, continual removal of waste.
