6.3.1 DNA Sequencin

Question 1

What is DNA sequencing?

Answer 1

• Allows organisms nucleotide base sequence to be observed

Question 2

Old to new DNA sequencing techniques

Answer 2

• Sanger sequencing 1970 (chain termination)
• High throughput sequencing (capillary electrophoresis and nanopore sequencing and pyrosequencing)

Question 3

What is a dideoxynucleotide?

Answer 3

• Chain termination method uses these
• Pair with complementary bases of nucleotides on template strand during replication
• When DNA polymerase encounters a dideoxynucleotide the developing strand stops developing

Question 4

What are primers?

Answer 4

Short strand of nucleotides with bases complementary to those at the start of the DNA fragment, and allow the copy strand to form which DNA polymerase can bind to

Question 5

What is the chain termination method?