6.2 Flashcards

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1
Q

suggest two purposes of plant collection in a modern botanic garden

A

-to protect rare species
-gene bank OR genetic resource/ store of alleles
-teaching/ education
-leisure/ amenity value/ aesthetic value

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2
Q

what is the role of PCR in sequencing a genome

A

-PCR amplifies DNA
-a range of different lengths of DNA- (chain terminating nucleotides)

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3
Q

what is the role of electrophoresis in sequencing a genome

A

-puts DNA pieces in size order
-to read base sequence/ order of basews

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4
Q

what is the role of digestion of DNA by restriction enzymes in sequencing a genome

A

-to cut (genome DNA) into smaller fragments
-to cut vectors/ plasmids

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5
Q

suggest why a genome has to be fragmented before sequencing

A
  • genome too big/ very large- would take too long
    -accuracy/ fewer errors with smaller fragments
    -divide job over time/ different labs
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6
Q

what are the benefits of genetically modified rice which have an increased vitamin A content

A

-reduces vitamin A deficiency
-reduce eye problems/ blindness

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7
Q

what are the ethical concerns of genetically modified rice which have an increased vitamin A content

A

-reduces genetic diversity/ variation
-clone may suffer from one disease/ environmental change
-spread of genes to other wild populations
-seeds expensive/ need to be brought every year
-rice does not grow in all areas where needed
-doubts whether vitamin A content is sufficient

-antibiotic gene transfer to pathogenic bacteria
-unknown effects/ cause mutation

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8
Q

what are the benefits of somatic gene therapy (genetically modified organism)

A

-cure/ reduce symptoms/ better Qol/ less medication
-cystic fibrosis/ Parkinson’s/ thalassaemia
-extend lifespan/ save lives

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9
Q

what are the ethical considerations of somatic gene therapy (genetically modified organism)

A

-virus vector may cause viral disease
-procedure may be invasive/ painful/ stressful
-temporary/ needs to be repeated/ limited success
-immune system/ rejection/ problems
-animal testing concerns

-antibiotic gene transfer to pathogenic bacteria
-unknown effects/ cause mutation

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