6. Gene probes and Genetic counselling

Question 1

define DNA probes

Answer 1

single stranded pieces of DNA which are complimentary to specific base sequences

Question 2

how can DNA probes be used

Answer 2

to highlight sequences on a gel i.e. fluorescent probes

Question 3

list the possible uses of DNA probes

Answer 3

• locate specific alleles of a gene
• see if a person is carrying the disease
• helps determine how patients will respond to drugs
• helps identify health risks

Question 4

outline the method of using a DNA probe

Answer 4

• restriction endonucleases digest the DNA sequence and is separated via gel electrophoresis
• DNA is incubated with a DNA probe
• if the complimentary DNA sequence is present the probe will join the specific base
• it is then put under a UV light, if the sequence is present the sample will glow

Question 5

name the possible uses of this technology

Answer 5

1. genetic counselling
2. personalised medicine