6

Question 1

Principle of virus neutralization test:

Answer 1

• Highly specific test based on the neutralisation of the virus by specific antibodies in serum tested
• Positive = serum contains specific antibodies that neutralise virus and virus can not damage the cells
• Negative = No antibodies, virus is not neutralised and can cause cytopathic effect

Question 2

Division of cell cultures according to the way of cultivation:
.a) Primary and established
.b) Monolayers and established
.c) Monolayers and suspension

Answer 2

.c) Monolayers and suspension

Question 3

Which medium is used for cultivation of fungi:
.a) Towenstein-Jenssen
.b) Hepatic agar
.c) Sabourad agar

Answer 3

.c) Sabourad agar

Question 4

Which staining method is used for microscopic detection of mycobacteria:
.a) Gram
.b) Koster
.c) Ziehl Nielsen

Answer 4

c) Ziehl Nielsen

Question 5

What are main reagent of PCR:

Answer 5

1. DNA
2. Two primers
3. Enzyme (Taq DNA polymerase)
4. dNTP
5. Reaction buffer
6. Termocycler

Question 6

Which is the gold standard test for laboratory diagnosis of rabies:
.a) Fluorescent antibody test
.b) Biological experiment
.c) Agglutination assay

Answer 6

.a) Fluorescent antibody test

Question 7

OIE is:
.a) Organisation of internal disease elimination
.b) World health organization
.c) World health organization for animal health

Answer 7

.c) World health organization for animal health

Question 8

Stages of infectious process:

Answer 8

• Incubation period
• Prodromal period
• Manifestation period
• Final period (recovery or death)

Question 9

What does the approach to one health mean:

Answer 9

• It recognises the interrelationship between animal, human and environmental health

Question 10

Source of infection is:
.a) Macroorganisms, in which etiological agent survives, multiplies and is excreted
.b) Macroorganisms, in which etiological agent survives and multiplies
.c) Macroorganisms, in which etiological agent survives and is excreted

Answer 10

.a) Macroorganisms, in which etiological agent survives, multiplies and is excreted