5.3 - Polypeptide Synthesis Flashcards
DNA in prokaryotes
Pro: circular DNA, a strand
* Located in the nucleoid, a region slightly denser than the rest of the cytoplasm
* Large circular simple chromosome - no histone proteins
* Sometimes accompanied by plasmids - smaller rings of DNA that contain limited genes
Plasmid
Provide selective advantages (antibiotics or resistance to external selective pressure)
It can be integrated into the main DNA structure if neded for replication
DNA in Eukaryotes
What is structure, and mtDNA
In eukaryotes, DNA is coiled in a histone protein framework chromosome
Often have DNA in organelles, such as mitochondria and the chloroplast.
A prominent example: mtDNA located in the mitochondria:
* mtDNA is formed in small circular rings genes resembling plasmids, genetic code for producing ATP.
* mtDNA is inherited from a single maternal lineage as all cytoplasmic organelles are inherited from the mother’s ovum (sperm has almost no cytoplasm). However, in forming a zygote half the nuclear DNA comes from the sperm and half from the ovum
Compare DNA in prokaryotes and eukaryotes
Prokaryotes:
* A circular chromosome with no telomeres
* Can be found in the form of plasmids, circular molecules (DNA is DNA not matter the organism)
* Less DNA than eukaryotes (thousands to millions of bases)
* Fewer genes (thousands)
* Less non-coding DNA (introns)
* Genes cluster into functional groups, operating together in operon regions
Eukaryotes:
* Linear thread-like with telomeres
* Formed only as a double helix
* More DNA (million to billion bases)
* More genes (tens of thousands)
* More introns
* Genes coding for functionally similar proteins can be physically apart or on different chromosomes. This is possible as they are more evolutionarily complex and can express these genes at the same time.
When answering this question, ensure that you discuss features of the DNA, not the cell features (organelles etc.) or location
Phenotype
examples to come
Expression of the genotype: physical appearance, physiology, behaviours.
It is determined partly by environment as it influences how a gene is expressed; the genetic makeup provides potential for traits, and environmental conditions such as diet, stress and temperature influence gene expression. Note: genes determine how phenotypes are expressed under genetic conditons.
Phenotype impacted by environment: flowers
Hydrangeas change colours based on the alkalinity.
In acidic conditions (below 6) aliminium ions are more available -> purple
Neutral to alkaline (above 6) aluminium ions are less available -> pink/red.
Genotype
Genetic code specific to an individual
Phenotype example: temperature-sensitive expression
There are 2
Himalayan rabbits
The Himalayan rabbit has a gene that codes for a pigment enzymes that is only active at cooler temperatures (below 20C). This enzyme is inactive at higher temperatures (above 30C). As the ears, nose, feet and tail are cooler than the rest of the body the enzyme is activated, resulting in darker pigmentation.
Australian bearded dragon lizard has genotypic sex determined by the presence of sex chromosomes. However, if eggs are incubated at high temepratures, genotypically male embryos will undergo sex-reversal into females. 2 genes are turned on under heat stress that override the sex chromosomes and trigger sex reversal
Proteins
A group of molecules that drive all processes in the body. Functions:
- Control all metabolic processes as enzymes
- Some send intracellular or intercellular signals (e.g. control release of insulin)
- Antibodies are involved in the adaptive immune system’s response to foreign pathogens in the body
- Transmembrane proteins alter cell membrane permeability (e.g. aquaporins and channel proteins)
- Some proteins build structure and mechanics of an organism, e.g. collagen and keratin
Proteins determine our phenotype with influence from the external environment
Phenotype example: alkalinity-sensitive expression
Hydrangeas
In acidic soil with pH < 6, aluminium ions in the soil become more available to the plant. The ions are absorbed, resulting in blue or purple flowers.
In neutral to alkaline soil ph 6+, aluminium ions are less accessible. The low absorption of these ions causes the flowers to be pink or red.
Amino acids
- Organic compounds containing amine and carboxyl functional groups, as well as a side chain (called an R group) which is determines the shape and structure of a polypeptide chain (aka a protein)
- Amino acids in a polypeptide chain are attracted to one another by intermolecular forces. Analogy: a necklace made of broken magnets. When released, the components will spontaneously form a particular shape.
- Shapes give the protein its specific role in the body.
Phenotype example: epigenetics
PKU disorder
It increases the levels of amino acid phenylalanine (phe) in the blood, and can lead to brain damage. The expression of phe can be prevented early in life if babies diagnosed with the PKU gene are placed on a continuously low-phe diet. This causes the PKU gene to not be expressed.
Secondary structure proteins
Local folded structures formed within a polypeptide due to interactions between atoms in the R-group
- 2 most common types of structures in this level are alpha-helix and beta-pleat
- Alpha-helix is a helical shape, single stranded unlike DNA
- Beta-pleat is like accordion folds
- These structures are held in shape by hydrogen bonds between the carbonyl O of one amino acid and the amino H of another.
Easy def: when sequence of amino acids are linked together by hydrogen bonds, forming alpha helix or beta pleats.
Tertiary structure proteins
Overall 3D structure of a polypeptide, occurs when certain attractions are present between alpha helics and pleated sheets
- Affected by interactions between R-groups of the amino acids that make up the protein
Protein structures
Polypeptides can form different structures
Primary, secondary, tertiary
