5. Properties of living things Flashcards

1
Q

what is metabolism?

A
  • catabolism + anabolism
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2
Q

what is important about metabolism?

A
  • all living organisms require carbon and energy to carry out metabolic process
  • classed according to carbon sources
    – autotroph
    – heterotroph
  • organisms require energy, hydrogen and electrons for growth
    – use radiant energy (phototrophs)
    – oxidation of organic/inorganic compounds (chemotrophs)
  • bacteria relying on inorganic energy sources and organic can be called mixotrophic
    – combine autotrophic and heterotrophic metabolic processes
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3
Q

give the different nutritional types, energy sources, carbon sources, and examples

A
  • photoautotrophs
    – light
    – CO2
    – microalgae
  • photoheterotrophs
    – light
    – organic compounds
    – microalgae
  • chemoautotrophs
    – chemicals
    – CO2
    – few bacteria, many archaea
  • chemoheterotrophs
    – chemicals
    – organic compounds
    – most bacteria, some archaea, fungi, yeasts
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4
Q

explain the differentiation of the bacterial endospore

A
  • number of gram +ve bacteria form special resistant dormant strucute (endospore) in response to stress
    – bacillus, clostridium, sporocarcina
  • resilience explained by unique structure
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5
Q

how is the strucutre of an endospore unique?

A
  • resistant to heat, UV radiation, chemical disinfectants, desiccation
  • important because not readil killed
    – antimicrobial treatments
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6
Q

what is the structure of an spore?

A
  • spore
    – can survive advers conditions for years
  • core
    – DNA, ribosomes, glycolitic enzymes
  • cytoplamsic membrane
  • spore wall
    – normal peptidoglycan
  • cortex
    – thick layer of less cross-linked peptidoglycan
  • keratin spore coat
    – protein
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7
Q

what is differentiation in swarmer and stalk cells?

A
  • single-celled bacterium undergoes cellular differentiation
    – Caulobacter crescentus
  • it differentiates into two cell types that do not co-operate
    – motile swarmer
    – stalk cells
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8
Q

what are the functions of the motile swarmer and stalk cells?

A
  • motile swarmer
    – find nutrient-rich place to attach and grow
    – loses flagellum, replacing with stalk that attaches to detritus in the pond
  • stalk cell
    – divides, then divides forming 2 daughter cells (a swarmer and a cell with original stalk)
    – swarmer cells swims to find nex location avoiding competitions with left stalk cell
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9
Q

how do cells communicate?

A
  • chemicals released and taken up
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10
Q

what is quorum sensing?

A
  • process of cell-to-cell communication allowing bacteria to share information about cell density and adjust gene expression accordingly
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11
Q

what does QS involve?

A
  • production, detection and response to extracellular signaling molecules
    – autoinducers (AIs)
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12
Q

how do AIs function?

A
  • accumulate in environment as population density increases
  • bacteria monitor this to track changes in cell number
  • collectively alter gene expression
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13
Q

what are the three basic principles of QS?

A
  • members of community produce AIs
    – at LCD AIs diffuse away
    –at HCD AIs lead to local high concentration enabling detection and response
  • AIs detected by receptors that exist in cytoplams or in membrane
  • in addition to acitivating gene expression, detection also results in activation of AI production
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14
Q

what is an example of QS?

A
  • Vibrio fischeri Lucl/LuxR QS circuit
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15
Q

how do squid use bioluminescent bacteria for survival?

A
  • bobtail squid has symbiotic relationship with bioluminescent Vibrio fischeri
  • V.fischeri colonises light producing organ
    – fed a sugar and amino acid solution by squid
    – serving as source of light
  • squid stay buried in sand during day
    – swim looking for food at night using organ on underside
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16
Q

how is QS used with V. fischeri?

A
  • high cell density
    – AIs of bioluminescence accumulate
  • leads to initiation of light emission vir regulation of lux operon
  • luxL and luxR genes that code for an accumulation or repression of AI’s causing for bioluminescence.
  • As the cell density is high, AI’s accumulate; where as in low cell density they diffuse out of the cell
    – thus regulating density by the squid eating or not eating the bioluminescent Vibrio. fischeri.