3. Composition of microbial world 2 Flashcards

1
Q

Prokaryotes

A
  • bacteria, archea
    – similar in size and shape
  • lack membrane-bound nucleus
  • lack membrane-bound organelles
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2
Q

Bacteria

A
  • prokaryotic
  • peptidoglycan cell walls
  • gram-positive; gram-negative
    – structure of cell wall
    – gram-negative can be antibiotic resistent
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3
Q

what are peptidoglycan cell walls

A
  • combination of peptide bonds & carbohydrates
    – peptide (amino acids)
    – glycan (sugars)
  • attacked by pennisylin
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4
Q

what are the arrangements of bacteria cells

A
  • coccus (spherical)
  • bacillus (rod shaped)
  • spirillium (sprial)
  • vibrio (crescent like)
  • spirochete
  • filamentous bacteria
  • cells will clump together if growth conditions are not good
    – formations depends on growth conditions
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5
Q

how to bacteria reproduce

A
  • binary fission
    – daughter cells identical to mother
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6
Q

how does binary fission work

A
  • replication of DNA
    – duplication of chromosome
  • cell growth
    – continued cell growth of cell
  • segregation of DNA
  • splitting of cells
    – division into two cells
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7
Q

what are archaea

A
  • prokaryotic
  • diverse physiology
  • range of single cell morphologies to filaments and even aggregates (0.1 - 15 micrometers)
  • hard to culture for biologists in lab conditions
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8
Q

how do archaea have diverse physiology

A
  • found to live in extreme environments
    – hot springs
    – geysers
    – gamma rays / UV radiation
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9
Q

what do archaea include

A
  • methanogens
  • extreme halophiles
  • extreme thermophiles
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10
Q

how do archaea differ from bacteria

A
  • cell wall structure
    – lacks peptidoglycan
  • unique membrane lipid structure
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11
Q

how is archaea membrane structure unique

A
  • unique lipid structure
  • side chains connected to glycerol backbone by ether bonds
    – instead of ester
  • side chains branched
    – phytanyl sidechain
    – sometimes form monolayer membrane
  • ether linkages vs. ester linkages
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12
Q

what are eukaryotes

A
  • multicellular / unicellular
  • true membrane-bound nuclei
  • DNA orgaises into chromosomes
  • cell reproductions by mitosis and meiosis
  • specific cellular functions in different organelles
    – eg. mitochondria respiration
    – eg. chloroplast photosythesis
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13
Q

how are eukaryotic cellular functions specific in different organelles?

A
  • specific cellular functions in different organelles
    – eg. mitochondria respiration
    – eg. chloroplast photosythesis
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14
Q

what are fungi

A
  • multi/unicellular
  • saprophytes
  • rigid cell walls (non-motile)
  • filamentous (hyphae)
  • secrete degradative enzymes into environments —- cellulases
    – proteases
    – amylases
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15
Q

what are fungi cell walls made up of

A
  • chitin
    – complex polysaccharide
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16
Q

what are fungi applications

A
  • pulp and paper industry
  • food industry (penicillium roqueforti - in cheese)
17
Q

what is yeast

A
  • unicellular
  • cell wall of chitin
  • asexual/sexual reproduction
18
Q

what are the applications of yeast

A
  • food industry
    – beer, cheese, bread
  • pharmaceutical industry
    – recombinant proteins
19
Q

what are microalgae

A
  • unicellular
  • asexual / sexual reproduction
  • photosynthetic
    – chloroplasts
    – sequester CO2
20
Q

what are the applications of microalgae

A
  • pharmaceutical
    – omega 3 fatty acids
  • biofuel
    – biodiesel
  • food industry
    –pigments
21
Q

what are viruses

A
  • acellular
  • DNA / RNA core
  • enclosed by coat of proteins
    – may be lipids
22
Q

what is the coat of viruses

A
  • coat of proteins
  • may be closed by lipid envelope
23
Q

how do viruses reproduce

A
  • only within living cells
24
Q

how are viruses classified

A
  • nucleic acids characteristics
  • capsid symmetry
  • their host
  • diseases they may cause
25
Q

how are the three domains of life compared?
Bacteria, Archaea, Eukarya:
- Nuclear envelope

A
  • absent
  • absent
  • present
26
Q

how are the three domains of life compared?
Bacteria, Archaea, Eukarya:
- Membrane-enclosed organelles

A
  • absent
  • absent
  • present
27
Q

how are the three domains of life compared?
Bacteria, Archaea, Eukarya:
- peptidoglycan in cell wall

A
  • present
  • absent
  • absent
28
Q

how are the three domains of life compared?
Bacteria, Archaea, Eukarya:
- membrane lipids

A
  • unbranched hydrocarbons
  • some branched hydrocarbons
  • unbranched hydrocarbons
29
Q

how are the three domains of life compared?
Bacteria, Archaea, Eukarya:
- RNA polymerase

A
  • one kind
  • several kinds
  • several kinds
30
Q

how are the three domains of life compared?
Bacteria, Archaea, Eukarya:
- Initiator amino acid for protein synthesis

A
  • formyl-methionine
  • methionine
  • methionine
31
Q

how are the three domains of life compared?
Bacteria, Archaea, Eukarya:
- introns in genes

A
  • very rare
  • present in some genes
  • present in many genes
32
Q

how are the three domains of life compared?
Bacteria, Archaea, Eukarya:
- Response to antibiotics streptomycin and chloramphenicol

A
  • growth inhibited
  • growth not inhibited
  • growth not inhibited
33
Q

how are the three domains of life compared?
Bacteria, Archaea, Eukarya:
- histones associated with DNA

A
  • absent
  • present in some species
  • present
34
Q

how are the three domains of life compared?
Bacteria, Archaea, Eukarya:
- circular chromosome

A
  • present
  • present
  • absent
35
Q

how are the three domains of life compared?
Bacteria, Archaea, Eukarya:
- Growth at temperatures >100 ‘C

A
  • no
  • some species
  • no
36
Q
A