5. Energy Transfers Flashcards
Photosynthesis
process in which light energy is converted into chemical energy in the form of glucose, symbol equation is 6CO2 + 6H2O + light energy —> C6H12O6 + 6O2
Chloroplast
organelle surrounded by a double membrane, thylakoids stacked into grana linked by lamellae providing a large surface area for the LDR, photosynthetic pigments which absorb light energy for the LDR, stroma containing enzymes for the LIR
Light-dependent reaction
first stage of photosynthesis, occurs in thylakoid membranes of chloroplast, involves photoionisation, chemiosmosis and photolysis, produces ATP and NADPH for the LIDR
Photoionisation
photosynthetic pigments (eg. chlorophyll) absorb light energy, exciting an electron which leaves the chlorophyll
Chemiosmosis
electron moves along the electron transport chain, releasing energy which is used to actively transport H+ into the thylakoid, forming a proton gradient, H+ diffuse down their electrochemical gradient into the stroma through ATP synthase joining ATP + Pi —> ATP
Photolysis
splitting of water producing H+, O2 and electrons, NADP picks up H+ and is reduced to NADPH
Light-independent reaction
second stage of photosynthesis, occurs in stroma of chloroplast, involves the Calvin cycle, produces useful organic compounds (eg. glucose)
Calvin cycle
CO2 combines with RuBP to form 2 molecules of GP, catalysed by the enzyme rubisco, both molecules of GP are reduced using 2ATP and 2NADPH to form 2 molecules of triose phosphate, most triose phosphate is regenerated into RuBP using energy from ATP, some triose phosphate is converted into useful organic compounds
Effect of light intensity
increasing light intensity means more light energy for the LDR, increasing rate of photosynthesis, light must be correct wavelength as different pigments absorb different wavelengths of light
Effect of temperature
increasing temperature means molecules have more kinetic energy, increasing rate of photosynthesis, beyond optimum enzymes denature, reducing rate
Effect of CO2 concentration
increasing CO2 concentration means more GP generated, increasing rate of photosynthesis, beyond optimum stomata close, reducing rate
Investigating photosynthetic pigments
add a spot of pigment onto the chromatography paper on the origin line, place the paper in solvent making sure solvent is below the origin line, remove before solvent reaches the top, calculate Rf value by dividing distance travelled by spot by distance travelled by solvent
Respiration
process in which chemical energy released from glucose is used to make ATP, symbol equation for aerobic respiration is C6H12O6 + 6O2 —> 6CO2 + 6H2O
Mitochondria
organelle surrounded by a double membrane, inner membrane folded into cristae providing a large surface area for oxidative phosphorylation, matrix containing enzymes for the link reaction and Krebs cycle
Glycolysis
takes place in the cytoplasm, glucose is phosphorylated using ATP into 2 molecules of triose phosphate, triose phosphate is oxidised into pyruvate, NAD is reduced into NADH, yields 2 pyruvate 2NADH and 2ATP
Link reaction
takes place in the mitochondria matrix, pyruvate is dehydrogenated and decarboxylated into acetate, acetate combines with coenzyme A to form acetyl coenzyme A, yields of 2 acetyl coenzyme A, 2CO2 and 2NADH (per glucose)
Krebs cycle
takes place in the mitochondria matrix, acetyl coenzyme A combines with a 4C compound to form a 6C compound, coenzyme A is recycled, 6C compound is dehydrogenated and decarboxylated into a 4C compound, NAD is reduced to NADH, FAD is reduced to FADH, ATP undergoes substrate level phosphorylation, yields 3NADH, 2CO2, FADH, ATP (per cycle)