5- Energy Transfers Flashcards
Where does the light dependent reaction occur
Thylakoid membranes and thylakoid spaces
What is the first stage of the light dependent reaction
Light absorption
Where is light energy absorbed by chlorophylls
Photosystems
What are photosystems made from
Accessory Pigments
What happens to the electrons when light energy is absorbed
The enter a more excited state and are released from the chlorophyll
What is the term used to describe chlorophyll, after the electrons have been released
Photoionized
What 3 reactions in photosyntheis are driven by the energy released from the photoionisation of chlorophylls
Photophosphorylation
Reduction
Photolysis
What is produced in photophosphorylation
ATP (from ADP and Pi)
Using Light energy and ETC
using proton gradient to drive ATP synthase
Where does photolysis occur
Thylakoid Lumen
What is the purpose of photolysis
to form a proton gradient between thylakoid lumen and stroma, as creates high conc of H+ in thylakoid lumen
In plants, where are the Electron carriers located?
Thylakoid Membranes
Where are the High energy electrons transferred to
Electron transport chain
How are electrons replaced in photosynthesis
the hydrolysis of water
How are H+ ions transported out of the thylakoid lumen
ATP synthase
what reaction does NADPH / reduced NADP get passed to
Light independent reaction
When electrons move down the ETC, they lose energy. What is this energy used for
To actively transport H+ from stroma into thylakoids through a proton pump, against their conc grad
What is the process of converting ADP –> ATP called, when using energy from protons diffusing through ATP synthase enzyme
Chemiosmosis
Some HEE are not passed along the ETC. Where do they get directly transferred to?
NADP
How do electrons transferred directly to NADP produce reduced NADP?
react with proton in stroma
What is produced in Non-cyclic phosphorylation
Reduced NADP and ATP
In non cyclic phosphorylation, which photosystem absorbes light to produce reduced NADP
PS1
What does not happen in non-cyclic phosphorylation
Electrons not recycled- , transferred from PSII to PSI
Which type of photophosphorylation only involves one photosystem, and which one is it?
Cyclic, photosystem 1
What happens to electrons in cyclic phosphorylation
cycle continously through electron carriers in PSI
What is chemiosmosis
Protons diffuse down conc grad across thylakoid mebrane through ATP synthase, releasing energy, which converts ADP + Pi –> ATP
What are the similarities and differences between Cyclic and Non-cyclic phosphorylation
Both produce ATP
Cyclic doesn’t produced NADP, Photlyisis doesn’t occur (as electrons continously recycled) so electrons in PSII aren’t replaced
Where does the light independent reaction (Calvin Cycle) occur
Stroma of chloroplasts
What does the calvin cycle use
ATP, reduced NADP, (from LDR)
CO2 and RuBP
What does the calvin cycle produce
Triose phosphates
Describe the first stage of the calvin cycle, giving its name
Carbon Fixation
CO2 diffuses into leaves, through stomata, and combines with RuBP -5C- producing an unstable 6C compound
Catalysed by rubisco
UNstable 6C splits into 2 molecules of GP- glycerate 3-phosphate, 3C
Describe the second stage of the calvin Cycle
Reduction of glycerate 3-phosphate
2 molecules of GP reduced to TP
reaction driven by Energy from 2 ATP, protons from 2 reduced NADP, both from LDR, recyled after use
1/6 TP molecules condense to hexose sugars (starch, sucrose, cellulose) converted to glycerol and GP (fatty acids, lipids (for cell membranes) ) used in production of some amino acids for protein synthesis
In the third stage of the calvin cycle, RuBP is regenerated. How does this occur
5/6 TP not converted, used to regenrate.
Driven by 1ATP
What are the three biomolecule products of the calvin cycle
Carbohydrates
Lipids
Amino Acids
Describe Cristae
Folded extensions of the inner membrane of the mitochondria,
less permeable than the outer mebrane
site of the ETC
location of ATP synthase
Give two conditions of the intermembrane space of the mitochondira
Low pH, due to a high conc of protons
Conc grad across inner membrane, formed during oxidative phosphorylation, essential for ATP synthase