5) Energy Transfer Flashcards
Chloroplasts
Site of photosynthesis
Chlorophyll in thylakoid membrane which is attached to proteins
Protein + pigment= photosystem
2 photosystems used to absorb light energy PSI and PSII
Electron transport chain- formed from photosystems + electron carriers
Stroma surrounds thylakoids
1) Light Dependent reaction of photosynthesis
1) light energy absorbed by PSII- excites electrons in chlorophyll. Chlorophyll photoionised
Electrons have more energy so can move along electron transport chain to PSI
2) Electrons that leave PSII- replaced through photolysis. Light splits water into H+, e- and oxygen.
3) Excited electrons lose energy as they move along the electron-transport chain. Energy used to transport H+ through thylakoid membrane so thylakoid has a higher concentration of H+ than stroma
H+ move down their concentration gradient into stroma via ATP synthase (chemiosomotic theory)
Energy from this combines ADP and phosphate—– ATP
4) Light energy absorbed by PSI- excites electrons to an even higher energy level
Electrons transferred to NADP with a H+ from stroma to form reduced NADP (used in light independent reaction)
2) Light independent reaction of photosynthesis
Calvin Cycle
1) Carbon dioxide diffuses to stroma- reacts with ribulose bisphosphate (RuBP) (5C)
= x2 glycerate 3-phosphate (GP) (3C)
Catalyzed by rubisco
2) Reduced NADP from light dependent reaction- reduces GP to triose phosphate (TP) (3C)
Uses energy supplied by ATP
3) Some TP——- useful organic substances (glucose)
Some TP used to regenerate RuBP (ATP needed)
Environmental factors that limit the rate of photosynthesis
Light
Temperature
Carbon dioxide
Cellular respiration definition
The formation of ATP from the breakdown of glucose
Overview of aerobic respiration
Requires oxygen
1) Glycolysis
2) Link reaction
3) Krebs cycle
4) Oxidative phosphorylation
Anaerobic respiration
X oxygen, produces less ATP
1 stage= glycolysis (same process as in aerobic respiration)
- Pyruvate produced in glycolysis can be converted to ethanol (plants) or lactate (animals) using reduced NAD
- Oxidised NAD produced can be used in further glycolysis
Stage 1 aerobic respiration: Glycolysis
Cytoplasm + produces pyruvate (3C)
1) Glucose phosphorylated- uses 1 ATP. = 1 glucose phosphate (6C) + 1 ATP
2) ATP used to add another phosphate = hexose bisphosphate (6C)
3) Hexose bisphosphate split into 2 triose phosphate (3C)
4) Triose phosphate is oxidized (lose Hydrogen) = 2 pyruvate (3C)- actively transported into matrix of mitochondria for link reaction
5) NAD is reduced by hydrogen atoms = 2 reduced NAD used in oxidative phosphorylation
6) 4 ATP produced, but 2 used up in phosphorylation in earlier stages so net gain of 2 ATP
Stage 2 aerobic respiration: Link reaction
Matrix of mitochondria, no ATP produced
1) pyruvate is decarboxylated (CO2 produced)
2) Pyruvate is oxidized to acetate- producing reduced NAD in the process
3) Acetate combines with coenzyme A (Co A) = acetyl coenzyme A (acetyl CoA)
2 pyruvate molecules are made for every glucose molecule that enter glycolysis. Means that link reaction + Krebs cycle occurs twice for every glucose molecule
Stage 3 aerobic respiration: Krebs cycle
Matrix of mitochondria. Series of oxidation-reduction reactions which generate reduced coenzymes (NAD and FAD) and ATP
1) Acetyl CoA + oxaloacetae (4C) ——— citrate (6C)
2) in a series of oxidation-reduction reactions, citrate loses carbon dioxide and hydrogen to give a 4 carbon molecule
3) ATP produced by direct transfer of a phosphate group to ADP by substrate level phosphorylation
Substrate level phosphorylation= when a phosphate group is directly transferred from one molecule to another
For each molecule of puyruvate, Krebs cycle produces reduced coenzymes (FAD and NAD), 1 ATP, 3 carbon dioxide
Stage 4 aerobic respiration: Oxidative phosphorylation
Energy carried by electrons, from reduced coenzymes, is used to make ATP. Electron transport chain, chemiosmosis
1) Hydrogen atoms released from reduced NAD and reduced FAD as they’re oxidized. H atoms split into protons and electrons
2) Electrons move down electron transport chain, losing energy at each electron carrier
3) Energy is used by electron carriers to pump protons from mitochondrial matrix into intermembrane space
4) Concentration of protons is now higher in intermembrane space than in mitochondrial matrix- electrochemical gradient
5) Protons move down electrochemical gradient, back across inner mitochondrial membrane + into mitochondrial matrix via ATP synthase- movement drives synthesis of ATP from ADP + phosphate
Chemiosmosis= process of ATP production driven by the movement of H+ ions across a membrane
What are the sources of carbon dioxide for plants in ecosystems?
atmospheric or aquatic carbon dioxide
Respiratory substrate definition
a substance required for cellular respiration to derive energy through oxidation
Structure of a food chain or web
Arrows show the direction of energy flow
Producers- photosynthetic organisms
Consumers- obtain energy by feeding on other organisms
Saprobionts- decomposers
Trophic level- each stage of the food chain
Biomass definition
Mass of an organism measured in terms of mass of carbon or dry mass of tissue per given area per given time